Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole
Cell Surface Display Fungal Laccase as a Renewable Biocatalyst for Degradation of Persistent Micropollutants Bisphenol A and Sulfamethoxazole
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DOI：
10.1021/acs.est.6b01641
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发表时间：
2016-08-16
影响因子：
11.4
通讯作者：
Wei, Na
中科院分区：
文献类型：
--
作者：
Chen, Yingying;Stemple, Brooke;Wei, Na
真菌漆酶具有较高的降解多种持久性有机污染物的活性。然而，在溶液中使用酶进行水处理具有不可重复使用、酶寿命短和一次性使用成本高的局限性。在这项研究中，我们利用合成生物学技术将真菌漆酶固定在酵母细胞表面，开发了一种新型生物催化剂。该生物催化剂被称为表面展示漆酶 (SDL)，其酶活性为 104 +/- 3 mU/g 干细胞（含有 2,2-azinobis-3-乙基苯并噻唑啉-6-磺酸盐 (ABTS)）。室温保存25天后，SDL保留了90%以上的初始酶活性；而相比之下，游离漆酶的活性下降至其初始活性的 60%。 SDL 可以高度稳定性地重复使用，因为在八次重复的批次反应后，它保留了 74% 的初始活性。使用双酚 A 和磺胺甲恶唑对 SDL 处理新出现的污染物的有效性进行了概念验证评估。污染物降解动力学的结果和氧化还原介体修正的影响提供了对影响 SDL 系统功效的因素的深入了解。这项研究首次报告了表面展示酶生物催化剂的开发，作为处理顽固性有机微污染物的有效且可再生的替代品。
Fungal laccases have high activity in degrading various persistent organic pollutants. However, using enzymes in solution for water treatment has limitations of nonreusability, short enzyme lifetimes, and high cost of Single use. In this study, we developed a new type of biocatalyst by immobilizing fungal laccase on the surface of yeast cells using synthetic biology techniques. The biocatalyst, referred to as surface display laccase (SDL), had an enzyme activity of 104 +/- 3 mU/g dry cell (with 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS)). The SDL retained over 90% of the initial enzyme activity after 25 days storage at room temperature; while, in contrast, activity of free laccase declined to 60% of its initial activity. The SDL could be reused with high stability as it retained 74% of initial activity after eight repeated batch reactions. Proof-Of-concept evaluations of the effectiveness of SDL in treating contaminants of emerging concern Were performed with bisphenol A and sulfamethoxazole. Results from contaminant degradation kinetics and the effects of redox mediator amendment provided insights into the factors affecting the efficacy of the SDL system. This study reports, for the first time, the development of a surface display enzyme biocatalyst as an effective and renewable alternative for treating recalcitrant organic micropollutants.