Identification and analysis of full-length transcripts involved in the biosynthesis of insecticidal lignan (+)-haedoxan A in Phryma leptostachya
Identification and analysis of full-length transcripts involved in the biosynthesis of insecticidal lignan (+)-haedoxan A in Phryma leptostachya
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细竹杀虫木脂素 ( )-haedoxan A 生物合成全长转录本的鉴定与分析
DOI：
10.1016/j.indcrop.2019.111868
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发表时间：
2019-12
影响因子：
5.9
通讯作者：
Hu Zhaonong
中科院分区：
文献类型：
--
作者：
Li Yankai;Wang Shaokang;Aioub Ahmed A.A.;Qie Xingtao;Wu Wenjun;Hu Zhaonong
Elucidating the biosynthetic pathway of bioactive ingredients will provide an excellent opportunity for their sustainable supply.Phryma leptostachya, a traditional insecticidal plant, is rich in lignans, among which (+)-haedoxan A (HA) is the major insecticidal ingredient, but its biosynthetic pathway remains unclear. Bioassay results showed that HA exhibited remarkably high insecticidal activity againstMythimna separatawith an LC50value of 26.67 mg/L, which was higher than that of 3 common botanical insecticides celangulin-V, rotenone, and pyrethrin, and approximate to that of 3 common chemical insecticides indoxacarb, chlorantraniliprole, and permethrin. The main accumulation space of HA and two other representative lignans (+)-phrymarolin I and II was localized in the roots ofP. leptostachyaby using high performance liquid chromatography (HPLC) and bioassay methods. A comparative transcriptome analysis was applied to identify and characterize the full-length transcripts of root, leaf, and stem tissues using a combination of next-generation sequencing (NGS) and single-molecule real-time (SMRT) sequencing, which provided a potential basis for further study the biosynthetic pathway of HA. This study is the first report on transcriptome analysis ofP. leptostachya, and 13 isoforms probably involved in the HA biosynthesis were finally collected. In conclusion, these results contribute to further research into the biosynthetic pathway of HA and other bioactive lignans, and the transcriptome data provide a valuable resource for investigating this crucial insecticidal plant.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
DOI：
10.1271/bbb.56.1751
发表时间：
1992-11
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
S. Yamauchi;E. Taniguchi
通讯作者：
S. Yamauchi;E. Taniguchi
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
长期读取的参考基因组无基因组的重建，从阿astagalusmembranaceus揭示了参与生物活性复合生物合成的转录物变体。
影响因子：
33.5
作者：
Li J;Harata-Lee Y;Denton MD;Feng Q;Rathjen JR;Qu Z;Adelson DL
通讯作者：
Adelson DL
影响因子：
3.7
作者：
Jung HJ;Cho YW;Lim HW;Choi H;Ji DJ;Lim CJ
通讯作者：
Lim CJ