Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication
Deletion of the cytoplasmic domain of CD163 enhances porcine reproductive and respiratory syndrome virus replication
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DOI：
10.1007/s00705-010-0699-8
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Lee, Changhee
中科院分区：
文献类型：
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作者：
Lee, Yoo Jin;Lee, Changhee
巨噬细胞清道夫受体CD163是猪繁殖与呼吸综合征病毒（PRRSV）进入机体的主要决定因素。本研究旨在评估CD163细胞内区域对PRRSV感染的功能意义。我们用编码全长和胞质结构域缺失（无尾）CD 163的质粒稳定转染非易感BHK细胞，并评估其对PRRSV的容许性。实验数据显示，无尾CD163不仅完全足以将非靶细胞转化为PRRSV易感性，而且还显著增强病毒复制，病毒蛋白合成和子代释放显著增加。总之，我们的研究结果表明，CD163的细胞内结构域可能与一个重要的尚未未知的功能在PRRSV感染。
The macrophage scavenger receptor CD163 is a major determinant for the entry of porcine reproductive and respiratory syndrome virus (PRRSV). The present study was undertaken to assess the functional significance of the intracellular region of CD163 for PRRSV infection. We stably transfected non-susceptible BHK cells with plasmids encoding full-length and cytoplasmic-domain-deleted (tailless) CD163 and evaluated their permissiveness to PRRSV. The experimental data revealed that the tailless CD163 was not only completely sufficient for converting non-target cells to PRRSV susceptibility, but it also notably enhanced virus replication, with significant increases in viral protein synthesis and progeny release. Taken together, our results suggest that the intracellular domain of CD163 may be associated with an important yet-unknown function during PRRSV infection.