Expression and immunohistochemical localization of vesicular glutamate transporter 2 in the migratory pathway from the rat olfactory placode
Expression and immunohistochemical localization of vesicular glutamate transporter 2 in the migratory pathway from the rat olfactory placode
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DOI：
10.1111/j.1460-9568.2004.03544.x
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发表时间：
2004-08-01
影响因子：
3.4
通讯作者：
Hisano, S
中科院分区：
文献类型：
--
作者：
Honma, S;Kawano, M;Hisano, S
通过免疫组织化学和原位杂交组织化学检查发育中的大鼠嗅觉区域中囊泡谷氨酸转运蛋白 2 (VGLUT2) 的定位，该定位与 NCAM（一种在分化神经元中表达的神经细胞粘附分子）和钙结合蛋白钙结合蛋白 D-28k（从犁鼻器官原基迁移的神经元标记物）的时空位置有特殊关系（Y. Toba 等，2017）。 (2001) J. Neuroendocrinol., 13, 683-694)。 VGLUT2 和 NCAM 免疫反应性首次在胚胎第 11.5 天 (E11.5) 在端脑囊泡下方的神经元细胞团中检测到。 E12.5后，在内侧和外侧嗅坑、犁鼻器原基和嗅上皮的迁移途径中检测到VGLUT2免疫反应性细胞。在 E15.5 和 E19.5 之间，在位于带有 NCAM 的犁鼻神经的细胞簇中观察到中度至强烈的 VGLUT2 免疫反应性，并且经常与钙结合蛋白 D-28k 免疫反应性共定位。使用原位杂交组织化学，在簇状细胞以及犁鼻和嗅上皮细胞中检测到 VGLUT2 mRNA 信号。 E20.5后，迁移细胞的数量逐渐减少，聚集的细胞中的VGLUT2免疫反应性减弱，尽管这些残留细胞中的钙结合蛋白D-28k免疫反应性仍然很强。从嗅觉基板主动迁移的神经元中存在强烈的 VGLUT2 免疫反应性，表明该转运蛋白参与了这些神经元的迁移过程。
The localization of vesicular glutamate transporter 2 (VGLUT2) was examined by immunohistochemistry and in situ hybridization histochemistry in the developing rat olfactory region with special relation to the spatiotemporal location of NCAM, a neural cell adhesion molecule expressed in differentiated neurons, and the calcium-binding protein calbindin D-28k, a marker of neurons migrating from the vomeronasal organ anlage (Y. Toba et al. (2001) J. Neuroendocrinol., 13, 683-694). Both VGLUT2 and NCAM immunoreactivities were first detected at embryonic day 11.5 (E11.5) in the neuronal cell mass beneath the telencephalic vesicle. After E12.5, VGLUT2-immunoreactive cells were detected in the migratory pathways from both medial and lateral olfactory pits, anlagen of the vomeronasal organ and olfactory epithelium. Between E15.5 and E19.5, moderate to intense VGLUT2 immunoreactivity was observed in cell clusters situated along NCAM-bearing vomeronasal nerves, and frequently colocalized with calbindin D-28k immunoreactivity. Using in situ hybridization histochemistry, VGLUT2 mRNA signals were detected in the clustered cells as well as in cells of the vomeronasal and olfactory epithelium. After E20.5, migrating cells gradually decreased in number and VGLUT2 immunoreactivity attenuated in the clustered cells, although calbindin D-28k immunoreactivity in these residual cells was still intense. The presence of intense VGLUT2 immunoreactivity in neurons actively migrating from the olfactory placode suggests that this transporter is involved in the migratory process of these neurons.