MEMBRANE BOUNDARIES INVOLVED IN THE UPTAKE AND INTRACELLULAR PROCESSING OF CELL-SURFACE RECEPTORS
MEMBRANE BOUNDARIES INVOLVED IN THE UPTAKE AND INTRACELLULAR PROCESSING OF CELL-SURFACE RECEPTORS
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DOI：
10.1016/0968-0004(86)90250-1
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发表时间：
1986-11-01
影响因子：
13.8
通讯作者：
HOPKINS, CR
中科院分区：
文献类型：
--
作者：
HOPKINS, CR
内分泌系统是一系列高度多态的膜结合亚区。大多数受体介导的研究从质膜下延伸到单核区。它在胞吞作用中起着重要作用，网格蛋白在处理内化的配体-受体复合物中起着重要作用，选择性地运输被膜凹坑作为受体将它们运送到细胞内各种目的地的位点。这些活动的意义集中在内化之前，调节受体和抗原的表面表达现在才成为第3步。现有的证据是显而易见的。内体系统与其他细胞内区室的关系与这样一种想法有关，即在这些内陷中，如高尔基体和溶酶体的内陷是复杂的，需要更清楚地确定，内化的膜蛋白以某种方式优先浓缩，而时间更长。大约十年前，许多实验室在过去的三年中，对细胞内膜进展感兴趣的质膜的稳定期居民已经被发现，但不包括细胞膜（见图1）。最近的研究膜抛开了它们对纯化的膜组分的排他性保留，继续剖析与外向的相互作用，这将允许组成生物合成途径之间的彻底的生化关系，并开始进行con-cal分析，并且仍在等待含网格蛋白的latcern分子本身与携带途径的体外模型系统的发展。同时，越来越多的迹象表明网格蛋白进入甚至穿过细胞.在1983年，人们普遍认识到它所占的争议份额，这些都是有选择性的。因此，目前的关注程度和重要性，这些途径已变得更加复杂的作用是集中在100和50 kDa的高潮，在一系列简短的评论，在介绍的术语，暗示排除组件，这被认为是谎言的大多数主线期刊。这些制品，将功能属性赋予更靠近脂质双层3的亚区室，尽管其包括系统内的串联呈现元件。考虑到这些组件在选择TIBSbyHeleniusetal中的预作用。landWillingham发送相当肤浅的水平之间的各种子集的蛋白质和Pastan 2，提供合成的证据，似乎是最在质膜仍然是来自各种各样的多形和塑料的所有细胞内定义的证据。最近的证据表明，来源，以确定一个共同的系统的ular膜系统，这似乎太大多数细胞含有一个池的游离网格蛋白5胞内隔室加工早期阶段采取严格的分类-和详细表征的配体-受体复合物内化。由于这个原因，术语“内未包被的ATP酶”能够从质膜上剥离。会用到焦点。目前，人们对组装网格9的兴趣似乎是包括所有的膜结合的元素，即网格蛋白网格是稳定的，而网格蛋白网格又包括了质膜细胞内的分子。尽管细胞内吞作用导致了细胞死亡，但迄今为止还没有蛋白质通过质膜的强制性选择性运输。在大多数细胞类型的证据表明，晶格积极出所有内膜边界，人口的内涵体…
The endosorne system is a highly pleomorphic series of membrane-bound subcompart- Most studies of receptor-mediated ments extending from below theplasma membrane to thejuxtanuclear area. Itplays an endocytosis have identified clathrin-essential role in processing internalized ligand-receptor complexes, selectively traffick-coated pits as the site at which receptors ing them to a variety of destinations within the cell. The significance of these activities are concentrated prior to internaliz-in regulating the surface expression of receptors and antigens are only now becoming ation 3. The available evidence is in keep-evident. The relationship of the endosome system to other intraceUular compartments ing with the idea that within these invagi-such as those of the Golgi and lysosome is complex and needs to be more clearly nations membrane proteins destined for defined, internalization are in some way preferentially concentrated while longer About ten years ago many laboratories During the last three years, steady term residents of the plasma membrane with an interest in intracellular mem-progress has been made but the preparaare excluded (see Fig. 1). Recent studies branes put aside their exclusive pre-tion of a purified membrane fraction have continued to dissect the interoccupation with outwardly directed which would allow thorough biochemi-relationships between the constituent biosynthetic pathways and began to con-cal analyses and the development of in molecules of the clathrin-containing latcern themselves with pathways carrying vitro model systems is still awaited. As it tice, with growing indications that clathproteins into, and even through, the cell. has progressed, the field has generated fin itself may not be an absolute necessity In 1983 a general appreciation of the its share of controversies and these have for selective uptake 4. Thus, current attenextentandimportanceofthesepathways been made more complicated by the tion is focused on the 100 and 50 kDa culminated in a series of brief reviews in introduction of terms that imply exclu-components which are thought to lie most main line journals. These articles, sive functional attributes to subcompart-closer to the lipid bilayer 3, although a which included a tandem presentation in ments within the system. Given the pre-role for these components in selecting TIBSbyHeleniusetal. landWillingham sent rather superficial level of under-between the various subsets of proteins and Pastan 2, provided syntheses of evi-standing of what appears to be the most in the plasma membrane remains to be dence derived from a wide variety of pleiomorphic and plastic of all intracell-defined. The recent demonstration that sources to identify a common system of ular membrane systems, this seems too most cells contain a pool of free clathrin 5 intracellular compartments processing early a stage to adopt rigid classifica-and the detailed characterization of an ligand-receptor complexes internalized tions. For this reason the term'endo-uncoating ATPase capable of disfrom the plasma membrane. The focus some'will be used. It is intended to assembling the lattice 9 argue against the of current interest now appears to be include all of the membrane-bound ele-view that clathrin lattices are stable leabroadening again to include the molec-ments involved in the intracellular pro-tures of the plasma membrane. Howular mechanisms responsible for the cessing that result from endocytosis at ever, there is as yet no compelling eviselective trafficking of proteins through-the plasma membrane. In most cell types dence to show that the lattice actively out all endomembrane boundaries, the population of endosomes …