Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1

Antibody Recognition Force Microscopy Shows that Outer Membrane Cytochromes OmcA and MtrC Are Expressed on the Exterior Surface of Shewanella oneidensis MR-1
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DOI:
10.1128/aem.02108-08
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发表时间:
2009-05-01
影响因子:
4.4
通讯作者:
Lower, Steven K.
Lower, Steven K.
中科院分区:
生物学2区
文献类型:
--
作者:
Lower, Brian H.;Yongsunthon, Ruchirej;Lower, Steven K.

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抗体识别力显微镜显示,当 Fe(III)(包括固相赤铁矿 (Fe(2)O(3)))为末端电子受体时,OmcA 和 MtrC 在活体 Shewanella oneidensis MR-1 细胞的外表面表达。 OmcA 定位于细胞和矿物质之间的界面。 MtrC 在细胞表面显示出更均匀的分布。两种细胞色素都与细胞外聚合物相关。
Antibody recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid-phase hematite (Fe(2)O(3)), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.