Analysis of cell cycle-related proteins in gastric intramucosal differentiated-type cancers based on mucin phenotypes: a novel hypothesis of early gastric carcinogenesis based on mucin phenotype.
Analysis of cell cycle-related proteins in gastric intramucosal differentiated-type cancers based on mucin phenotypes: a novel hypothesis of early gastric carcinogenesis based on mucin phenotype.
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DOI：
10.1186/1471-230x-10-55
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发表时间：
2010-06-07
影响因子：
2.4
通讯作者：
Suzuki K
中科院分区：
文献类型：
--
作者：
Sugai T;Tsukahara M;Endoh M;Shioi Y;Takebe N;Mue Y;Matsushita H;Toyota M;Suzuki K
细胞周期调节因子的缺失是人类癌症的共同特征，其中一些因子与胃癌的早期发展有关。然而，最近的研究表明，胃癌的发生与粘蛋白的表达有关。因此，在分化型胃癌的早期阶段研究细胞周期相关蛋白的表达模式，以确定与粘蛋白表型的任何机制关系。使用细胞周期蛋白D1、A、E以及p21、p27、p53和β-连环蛋白的免疫染色来检查190例胃粘膜内分化型癌症的细胞周期损伤。通过MUC 5AC、MUC 6、MUC 2和CD 10的表达来确定粘蛋白表型。Ki-67阳性率（PR）也进行了检查。p53、细胞周期蛋白D1和细胞周期蛋白A的过度表达在胃表型中比在肠表型中更频繁。与肠表型相比，细胞周期蛋白A在混合表型中过表达，而p27过表达在肠表型中比在混合表型中更频繁。p21的减少是胃粘膜内分化型癌的共同特征。我们的研究结果表明，一些细胞周期调节因子的水平似乎与早期胃分化型癌的粘蛋白表型。
Abnormalities of cell cycle regulators are common features in human cancers, and several of these factors are associated with the early development of gastric cancers. However, recent studies have shown that gastric cancer tumorigenesis was characterized by mucin expression. Thus, expression patterns of cell cycle-related proteins were investigated in the early phase of differentiated-type gastric cancers to ascertain any mechanistic relationships with mucin phenotypes. Immunostaining for Cyclins D1, A, E, and p21, p27, p53 and β-catenin was used to examine impairments of the cell cycle in 190 gastric intramucosal differentiated-type cancers. Mucin phenotypes were determined by the expressions of MUC5AC, MUC6, MUC2 and CD10. A Ki-67 positive rate (PR) was also examined. Overexpressions of p53, cyclin D1 and cyclin A were significantly more frequent in a gastric phenotype than an intestinal phenotype. Cyclin A was overexpressed in a mixed phenotype compared with an intestinal phenotype, while p27 overexpression was more frequent in an intestinal phenotype than in a mixed phenotype. Reduction of p21 was a common feature of the gastric intramucosal differentiated-type cancers examined. Our results suggest that the levels of some cell cycle regulators appear to be associated with mucin phenotypes of early gastric differentiated-type cancers.