The JAK/STAT pathway is required for border cell migration during Drosophila oogenesis
The JAK/STAT pathway is required for border cell migration during Drosophila oogenesis
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DOI：
10.1016/s0925-4773(01)00615-3
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发表时间：
2002-02-01
影响因子：
2.6
通讯作者：
Rorth, P
中科院分区：
文献类型：
--
作者：
Beccari, S;Teixeira, L;Rorth, P
在果蝇卵子发生过程中，边缘细胞进行刻板的迁移。Slbo是一种C/EBP转录因子，是这种迁移所必需的。在这里，我们确定果蝇Stat 92 E（信号转导和转录激活子）在屏幕上的功能获得性抑制的slbo突变体表型。通过对Stat 92 E和hop（果蝇JAK）突变体的克隆分析，我们发现JAK/STAT途径是边缘细胞迁移所必需的。该通路的活化配体Unpaired在极性细胞中表达。极细胞是毛囊前部细胞中能诱导边缘细胞命运的特化细胞。就其本身而言，未配对的异位表达可诱导包括Slbo的边缘细胞标志物的异位表达。然而，Stat 92 E突变细胞仍然表达正常水平的Slbo蛋白，因此Stat 92 E必须调节对边界细胞迁移至关重要的其他靶标。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
During Drosophila oogenesis, border cells perform a stereotypic migration. Slbo, a C/EBP transcription factor, is required for this migration. Here we identify Drosophila Stat92E (signal transducer and activator of transcription) in a screen for gain-of-function suppressors of the slbo mutant phenotype. By clonal analysis for Stat92E and hop (Drosophila JAK) mutants we find that the JAK/STAT pathway is required in border cells for their migration. The activating ligand for the pathway, Unpaired, is expressed in polar cells. Polar cells are specialized cells which can induce border cell fate in anterior follicle cells. On its own, ectopic expression of Unpaired can induce ectopic expression of border cell markers including Slbo. However, Stat92E mutant cells still express normal levels of Slbo protein, thus Stat92E must regulate other targets critical for border cell migration. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.