Cadmium metallothionein gene of Tetrahymena pyriformis.
Cadmium metallothionein gene of Tetrahymena pyriformis.
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DOI：
10.1016/s0378-1119(99)00181-x
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发表时间：
1999-06
期刊：
影响因子：
3.5
通讯作者：
E. Piccinni;D. Bertaggia;G. Santovito;C. Miceli;A. Kraev
中科院分区：
文献类型：
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作者：
E. Piccinni;D. Bertaggia;G. Santovito;C. Miceli;A. Kraev
从梨形四膜虫，编码镉诱导的金属硫蛋白的基因组序列已被克隆。该基因编码487个碱基的转录本，具有324个核苷酸的无内含子编码区，以TGA为终止密码子，TAA编码谷氨酰胺，翻译起始序列为AAAATGG。两个区域的内部相似性的编码序列支持的假设，四膜虫蛋白质产生的基因复制。非翻译区序列与线虫MT-1和MT-2转录本序列有一定的相似性。转录起始点上游525个碱基的序列包含一个TATA盒、一个CAAT盒反向互补序列和许多与AP-1和ACE-1结合位点部分匹配的短片段，但没有发现其他金属硫蛋白启动子的特征序列。
A genomic sequence from Tetrahymena pyriformis, encoding a cadmium-induced metallothionein has been cloned. The gene encodes a transcript of 487bases, with an intronless coding region of 324 nt, using TGA as the stop codon, TAA coding for glutamine, and the translational initiation sequence AAAATGG. Two regions of internal similarity in the coding sequence support the hypothesis that the Tetrahymena protein arose by gene duplication. The sequences of untranslated regions show some similarities with nematode MT-1 and MT-2 transcripts. Sequence of 525bases upstream of the transcription start contains a TATA box, a CAAT box reverse complement, and many short stretches partially matching the AP-1 and ACE-1 binding sites, but no characteristic sequences found in other metallothionein promoters.