Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium
Tissue tension and not interphase cell shape determines cell division orientation in the Drosophila follicular epithelium
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DOI：
10.15252/embj.2018100072
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发表时间：
2019-02-01
期刊：
影响因子：
11.4
通讯作者：
Bergstralh, Dan T.
中科院分区：
文献类型：
--
作者：
Finegan, Tara M.;Na, Daxiang;Bergstralh, Dan T.
我们研究了果蝇卵泡上皮在早期卵室扩张和伸长过程中的形态发生。我们发现细胞分裂不是早期卵泡上皮伸长所必需的，而是驱使组织向最佳几何包装方向发展。我们检查了相对于平面组织轴的细胞分裂方向，发现偏向于组织扩张的主要方向。然而，间期细胞的形状表现出相反的偏见。因此，认为细胞伸长决定分裂方向的Hertwig法则在这个组织中被打破了。这一观察结果不能用保守的Pins/Mud纺轴定向机制的各向异性活性来解释，该机制控制着顶基轴的分裂方向和其他上皮组织的平面分裂方向。相反，顶端表面的皮质张力以一种依赖于Canoe/Afadin的方式转化为平面分裂方向，这将肌动球蛋白与粘附连接联系起来。这些发现表明，在增殖上皮中，不同轴的分裂方向——尖-基轴和平面轴——是由不同的独立机制控制的。
We investigated the cell behaviors that drive morphogenesis of the Drosophila follicular epithelium during expansion and elongation of early-stage egg chambers. We found that cell division is not required for elongation of the early follicular epithelium, but drives the tissue toward optimal geometric packing. We examined the orientation of cell divisions with respect to the planar tissue axis and found a bias toward the primary direction of tissue expansion. However, interphase cell shapes demonstrate the opposite bias. Hertwig's rule, which holds that cell elongation determines division orientation, is therefore broken in this tissue. This observation cannot be explained by the anisotropic activity of the conserved Pins/Mud spindle-orienting machinery, which controls division orientation in the apical-basal axis and planar division orientation in other epithelial tissues. Rather, cortical tension at the apical surface translates into planar division orientation in a manner dependent on Canoe/Afadin, which links actomyosin to adherens junctions. These findings demonstrate that division orientation in different axes-apical-basal and planar-is controlled by distinct, independent mechanisms in a proliferating epithelium.