HUMAN MYOBLAST TRANSPLANTATION - PRELIMINARY-RESULTS OF 4 CASES
HUMAN MYOBLAST TRANSPLANTATION - PRELIMINARY-RESULTS OF 4 CASES
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DOI：
10.1002/mus.880150504
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发表时间：
1992-05-01
期刊：
影响因子：
3.4
通讯作者：
TREMBLAY, JP
中科院分区：
文献类型：
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作者：
HUARD, J;BOUCHARD, JP;TREMBLAY, JP
来自免疫相容供体的成肌细胞已被移植到4例晚期Duchenne患者的肌肉（胫骨前肌、肱二头肌和/或桡腕长伸肌）中。虽然没有使用免疫抑制治疗，但没有患者出现任何临床排斥症状，如发烧、发红和炎症。通过细胞荧光分析，一名患者短暂产生了针对供体成肌细胞的抗体。这名患者和另外两名患者显示出对其供体的肌管产生抗体。4例患者注射后的胫骨前肌肌肉活检显示，分别有80%、75%、25%和0%的肌纤维出现不同程度的肌营养不良蛋白免疫染色。后3例患者对侧未注射肌肉未见肌营养不良蛋白阳性纤维，而第1例患者对侧肌营养不良蛋白表达率为16%。成肌细胞也被注射到这些患者的桡侧腕长伸肌或肱二头肌。注射几个月后，一名患者在静态手腕伸展时的力量增加了143%。该结果必须谨慎解释，因为没有使用双盲强度测量方案。此外，我们注意到这种变化随着时间的推移而缓慢衰减。另外2例患者强度增加不明显（41%和51%），而第4例患者强度不变。
Myoblasts from immunocompatible donors have been transplanted into the muscles (tibialis anterior, biceps brachii, and/or extensor carpi radialis longus) of 4 Duchenne patients in the advanced stages of the disease. Although no immunosuppressive treatment was used, none of the patients showed any clinical signs of rejection such as fever, redness, and inflammation. One patient transiently produced antibodies against the donor myoblasts as determined by cytofluorometric analysis. This patient and 2 others were shown to form antibodies against their donor's myotubes. Muscle biopsies of the injected tibialis anterior of 4 patients revealed that 80%, 75%, 25%, and 0% of the muscle fibers, respectively, showed some degree of dystrophin immunostaining. The contralateral noninjected muscles of the latter 3 patients did not contain any dystrophin positive fibers, while that of the first patient showed dystrophin expression in 16% of the fibers examined. Myoblasts were also injected into the extensor carpi radialis longus or the biceps brachii of these patients. A few months subsequent to injection, one patient was shown to have a 143% increase of strength during static wrist extension. This result must be interpreted with caution because a double-blind strength-measuring protocol was not used. Furthermore, we have noted that this change slowly decayed over time. The strength of 2 other patients was increased less remarkably (41% and 51%), while the strength of the fourth patient was unchanged.