Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase.
Specific interactions among transmembrane 4 superfamily (TM4SF) proteins and phosphoinositide 4-kinase.
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DOI：
10.1042/bj3510629
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发表时间：
2000-11
期刊：
影响因子：
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通讯作者：
Robert L. Yauch;M. Hemler
中科院分区：
文献类型：
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作者：
Robert L. Yauch;M. Hemler
在早期的工作中，我们确定磷酸肌醇 4-激酶（PI 4-激酶）可能与跨膜 4 超家族（TM4SF，四跨膜蛋白）蛋白相关，但关键的特异性问题并未得到解决。在这里，我们证明至少五种不同的 TM4SF 蛋白（CD9、CD63、CD81、CD151 和 A15/TALLA1）可以与相似或相同的 55 kDa II 型 PI 4 激酶结合。这些关联是特定的，因为我们没有发现其他磷酸肌醇激酶（例如磷酸肌醇 3-激酶和磷酸肌醇-4-磷酸 5-激酶）与 TM4SF 蛋白关联的证据，并且许多其他 TM4SF 蛋白（包括 CD82 和 CD53）不与 PI 4-激酶关联。 CD63-PI 4-激酶复合物几乎完全在细胞内，因此与主要位于质膜中的其他 TM4SF-PI 4-激酶复合物（例如涉及 CD9）不同。这些结果表明，TM4SF 蛋白的特定子集可能会将 PI 4 激酶募集到特定的膜位置，从而影响磷酸肌醇依赖性信号传导。
In earlier work we established that phosphoinositide 4-kinase (PI 4-kinase) may associate with transmembrane 4 superfamily (TM4SF, tetraspanin) proteins, but critical specificity issues were not addressed. Here we demonstrate that at least five different TM4SF proteins (CD9, CD63, CD81, CD151 and A15/TALLA1) can associate with a similar or identical 55 kDa type II PI 4-kinase. These associations were specific, since we found no evidence for other phosphoinositide kinases (e.g. phosphoinositide 3-kinase and phosphoinositide-4-phosphate 5-kinase) associating with TM4SF proteins, and many other TM4SF proteins (including CD82 and CD53) did not associate with PI 4-kinase. CD63-PI 4-kinase complexes were almost entirely intracellular, and thus are distinct from other TM4SF-PI 4-kinase complexes (e.g. involving CD9), which are largely located in the plasma membrane. These results suggest that a specific subset of TM4SF proteins may recruit PI 4-kinase to specific membrane locations, and thereby influence phosphoinositide-dependent signalling.