Characterization of mitochondrial injury after cardiac arrest (COMICA)
Characterization of mitochondrial injury after cardiac arrest (COMICA)
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DOI：
10.1016/j.resuscitation.2016.12.029
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发表时间：
2017-04-01
期刊：
影响因子：
6.5
通讯作者：
Callaway, Clifton W.
中科院分区：
文献类型：
--
作者：
Donnino, Michael W.;Liu, Xiaowen;Callaway, Clifton W.
简介：心脏骤停后的线粒体损伤已在临床前环境中描述，但这种损伤在人类中发生的程度在很大程度上仍然未知。我们假设，线粒体生物标志物的水平增加将与死亡率和神经系统发病率在心脏骤停后subjects.Methods：我们进行了一项前瞻性多中心研究心脏骤停后的科目。入选标准为患有院外心脏骤停的昏迷成年人。线粒体生物标志物在恢复自主循环后0，12，24，36和48 h以及在健康controls.Results：出111例受试者入组，102在0 h的可评估的样品进行了测量。与对照组相比，心脏骤停受试者的基线细胞色素c水平较高（2.18 ng/mL [0.74，7.74] vs. 0.16 ng/mL [0.03，0.91]，p < 0.001），死亡受试者的0 h细胞色素c水平高于幸存者（3.66 ng/mL [1.40，14.9]对比1.27 ng/mL [0.16，2.37]，p < 0.001）。与对照组受试者相比，心脏骤停受试者基线时核糖核酸酶P（RNaseP）（3.3 [1.2，5.7] vs. 1.2 [0.8，1.2]，p < 0.001）和β 2微球蛋白（B2 M）（12.0 [1.0，22.9] vs. 0.6 [0.6，1.3]，p < 0.001）水平显著较高。幸存者和非幸存者的线粒体DNA，核DNA，或细胞游离DNA.Conclusions：细胞色素C增加心脏骤停后的受试者相比，对照组，并在心脏骤停后非幸存者相比，幸存者。心脏骤停后血浆中细胞核DNA和游离DNA含量增加。存活者和非存活者之间的线粒体DNA、核DNA或游离DNA没有差异。线粒体损伤标志物在心脏骤停后显示了混合结果。未来的研究需要研究这些差异。(C)2017爱思唯尔B. V.保留所有权利。
Introduction: Mitochondrial injury post-cardiac arrest has been described in pre-clinical settings but the extent to which this injury occurs in humans remains largely unknown. We hypothesized that increased levels of mitochondrial biomarkers would be associated with mortality and neurological morbidity in post-cardiac arrest subjects.Methods: We performed a prospective multicenter study of post-cardiac arrest subjects. Inclusion criteria were comatose adults who suffered an out-of-hospital cardiac arrest. Mitochondrial biomarkers were measured at 0, 12, 24, 36 and 48 h after return of spontaneous circulation as well as in healthy controls.Results: Out of 111 subjects enrolled, 102 had evaluable samples at 0 h. Cardiac arrest subjects had higher baseline cytochrome c levels compared to controls (2.18 ng/mL [0.74, 7.74] vs. 0.16 ng/mL [0.03, 0.91], p < 0.001), and subjects who died had higher 0 h cytochrome c levels compared to survivors (3.66 ng/mL [1.40, 14.9] vs. 1.27 ng/mL [0.16, 2.37], p < 0.001). There were significantly higher Ribonuclease P (RNaseP) (3.3 [1.2, 5.7] vs. 1.2 [0.8, 1.2], p < 0.001) and Beta-2microglobulin (B2M) (12.0 [1.0, 22.9], vs. 0.6 [0.6, 1.3], p < 0.001) levels in cardiac arrest subjects at baseline compared to the control subjects. There were no differences between survivors and non-survivors for mitochondrial DNA, nuclear DNA, or cell free DNA.Conclusions: Cytochrome c was increased in post-cardiac arrest subjects compared to controls, and in post-cardiac arrest non-survivors compared to survivors. Nuclear DNA and cell free DNA was increased in plasma of post-cardiac arrest subjects. There were no differences in mitochondrial DNA, nuclear DNA, or cell free DNA between survivors and non-survivors. Mitochondrial injury markers showed mixed results in the post-cardiac arrest period. Future research needs to investigate these differences. (C) 2017 Elsevier B.V. All rights reserved.