Black Phosphorus Nanosheets Immobilizing Ce6 for Imaging-Guided Photothermal/Photodynamic Cancer Therapy
Black Phosphorus Nanosheets Immobilizing Ce6 for Imaging-Guided Photothermal/Photodynamic Cancer Therapy
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DOI：
10.1021/acsami.8b00276
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发表时间：
2018-04-18
影响因子：
9.5
通讯作者：
Dong, Xiaochen
中科院分区：
文献类型：
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作者：
Yang, Xiaoyan;Wang, Dongya;Dong, Xiaochen
在预防和临床研究中，消灭肿瘤，特别是深部肿瘤，仍然是一个巨大的挑战。光动力学疗法和光热疗法是治疗组织癌的有前途的替代方法。黑磷（BP）基纳米材料具有宽的紫外-可见近红外吸收和优异的光热效应，在生物医学领域显示出巨大的应用潜力。在此，已经开发了一种生物相容性治疗平台，二氢卟酚e6（Ce 6）修饰的BP纳米片（NS），用于荧光和热成像引导的光热和光动力协同癌症治疗。利用剥离的BP NS的相对高的表面积，PEG-NH 2改性的BP NS（BP@PEG）负载有Ce 6光敏剂。结果表明，BP@PEG/Ce 6纳米结构不仅具有良好的生物相容性、生理稳定性和肿瘤靶向性，而且与BP@PEG纳米结构（28.7%）相比，其光热转换效率（43.6%）也有明显提高。此外，由于Ce 6光敏剂的释放，BP@PEG/Ce 6 NS可有效地产生活性氧，这也被体外研究所证实。体内荧光成像表明，BP@PEG/Ce 6 NS可通过增强的渗透性和滞留效应在肿瘤中靶向蓄积。体外和体内研究表明，BP@PEG/Ce 6可以是一种有前途的协同光热/光动力癌症治疗的纳米治疗剂。
In predinical and clinical research, to destroy cancers, particularly those located in deep tissues, is still a great challenge. Photodynamic therapy and photothermal therapy are promising alternative approaches for tissue cancer curing. Black phosphorus (BP)-based nanomaterials, with broad UV-vis near-infrared absorbance and excellent photo thermal effect, have shown great potential in biomedical applications. Herein, a biocompatible therapeutic platform, chlorin e6 (Ce6)-decorated BP nanosheets (NSs), has been developed for fluorescence and thermal imaging-guided photothermal and photodynamic synergistic cancer treatment. Taking advantage of the relatively high surface area of exfoliated BP NSs, the PEG-NH2-modified BP NSs (BP@PEG) are loaded with a Ce6 photosensitizer. The resulted BP@PEG/Ce6 NSs not only have good biocompatibility, physiological stability, and tumor-targeting property but also exhibit enhanced photothermal conversion efficiency (43.6%) compared with BP@PEG NSs (28.7%). In addition, BP@PEG/Ce6 NSs could efficiently generate reactive oxygen species because of the release of the Ce6 photosensitizer, which is also verified by in vitro studies. In vivo fluorescence imaging suggests that BP@PEG/Ce6 NSs can accumulate in the tumor targetedly through the enhanced permeability and retention effect. Both in vitro and in vivo studies suggest that BP@PEG/Ce6 can be a promising nanotheranostic agent for synergetic photothermal/photodynamic cancer therapy.