IMMUNE-RESPONSES OF SPECIFIC PATHOGEN-FREE AND GNOTOBIOTIC MICE TO ANTIGENS OF INDIGENOUS AND NONINDIGENOUS MICROORGANISMS
IMMUNE-RESPONSES OF SPECIFIC PATHOGEN-FREE AND GNOTOBIOTIC MICE TO ANTIGENS OF INDIGENOUS AND NONINDIGENOUS MICROORGANISMS
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DOI：
10.1128/iai.11.2.320-329.1975
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
SAVAGE, DC
中科院分区：
文献类型：
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作者：
BERG, RD;SAVAGE, DC
从SPF （specific pathogen-free， SPF）小鼠的胃肠道中分离到本地大肠杆菌、拟杆菌和乳杆菌。未接种SPF疫苗的小鼠在其脾脏中显示出少量的斑块形成细胞（PFC）和玫瑰花形成细胞与这些原生源细菌的抗原反应。直到出生后7天，才在SPF小鼠中检测到PFC与这些细菌抗原的反应。与未接种疫苗的对照组相比，SPF小鼠经肠外接种了本地大肠杆菌或拟杆菌后，特异性pfc的数量适度增加。因此，SPF小鼠在接种肠道本地微生物后能够产生免疫应答。然而，与接种本地大肠杆菌或拟杆菌相比，接种非本地大肠杆菌O127:B8、大肠杆菌O14或脆弱杆菌后，SPF小鼠与同源疫苗抗原反应的PFC更多。口服与这些非本地细菌单相关的灵生菌小鼠比与本地微生物单相关的灵生菌对用于单关联的细菌抗原表现出更大的免疫反应。结果与假设一致，即小鼠对非本地细菌抗原的免疫反应比它们对胃肠道某些本地微生物抗原的免疫反应更强。
Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous bacteria. PFC reacting with these bacterial antigens were not detected in infant SPF mice until 7 days after birth. Compared with nonvaccinated controls, SPF mice vaccinated parenterally with indigenous E. coli or Bacteroides produced a moderate increase in the numbers of specific PFC. Thus, the SPF mouse is capable of responding immunologically after vaccination with microbes indigenous to its intestinal tract. However, more PFC reacting with homologous vaccine antigens were detected after parenteral vaccination of SPF mice with nonindigenous E. coli O127:B8, E.coli O14, or B. fragilis than after parenteral vaccination with indigenous E. coli or Bacteroides. Gnotobiotic mice orally monoassociated with these nonindigenous bacteria exhibited greater immune responses to antigens of the bacteria used to monoassociation than did gnotobiotes monoassociated with the indigenous microbes. The results are consistent with the hypothesis that mice are more responsive immunologically to antigens of nonindigenous bacteria than they are to antigens of certain microbes indigenous to their gastrointestinal tracts.