VOLATILE CARBONYL LEVELS IN TISSUES OF TRANSGENIC MICE WITH NERVE SHEATH TUMORS
VOLATILE CARBONYL LEVELS IN TISSUES OF TRANSGENIC MICE WITH NERVE SHEATH TUMORS
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DOI：
10.1016/0378-4347(93)e0436-t
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发表时间：
1994-03-18
期刊：
影响因子：
--
通讯作者：
SHIBAMOTO, T
中科院分区：
文献类型：
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作者：
EBELER, SE;HINRICHS, SH;SHIBAMOTO, T
测定了荷瘤转基因小鼠不同组织匀浆中挥发性羰基化合物的含量。甲醛和乙醛被衍生为噻唑烷类化合物。丙二醛被衍生为1-甲基吡唑。用具有高灵敏度和特异性的氮磷检测器的气相色谱对这些衍生物进行定量。甲醛和丙二醛的定量限分别为2微克/毫升和27微克/毫升。携带肿瘤的转基因小鼠的红细胞和腓肠肌中的丙二醛水平比对照非转基因小鼠的相同组织中高。荷瘤小鼠的脑、肝、肾、心脏和脾组织丙二醛水平降低。甲醛和乙醛也得到了类似的结果。
Volatile carbonyl compounds in homogenates prepared from various tissues of tumor-bearing transgenic mice were determined. Formaldehyde and acetaldehyde were derivatized to thiazolidines. Malonaldehyde was derivatized to 1-methylpyrazole. The derivatives were quantified by gas chromatography with a highly sensitive and specific nitrogen-phosphorus detector. The limits of quantitation of formaldehyde and malonaldehyde were 2 mug/ml of homogenate and 27 ng/ml of homogenate, respectively. Levels of malonaldehyde in the erythrocytes and gastrocnemius of tumor-bearing transgenic mice were elevated as compared to the same tissue in control non-transgenic mice. Brain, liver, kidney, heart, and spleen tissues of the tumor-bearing mice exhibited decreased malonaldehyde levels. Similar results were obtained for formaldehyde and acetaldehyde.