Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset
Chronic Implantation of Whole-cortical Electrocorticographic Array in the Common Marmoset
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DOI：
10.3791/58980
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发表时间：
2019-02-01
影响因子：
1.2
通讯作者：
Ichinohe, Noritaka
中科院分区：
文献类型：
--
作者：
Komatsu, Misako;Kaneko, Takaaki;Ichinohe, Noritaka
脑电地形图(ECoG)能够以高时空分辨率监测大脑皮层的电场电位。最近开发的薄而灵活的ECoG电极使大规模皮质活动的稳定记录成为可能。我们研制了一种针对普通绒猴的全皮质皮层皮层脑电阵列。该阵列连续覆盖几乎整个皮质半球的外侧表面，从枕极到颞极和额极，它在一次拍摄中捕捉到整个大脑皮层的神经活动。该方案描述了阵列在绒猴脑硬膜外间隙的慢性植入过程。绒猴在ECoG记录方面有两个优势，其一是人类和猕猴的解剖结构的同源组织，包括额叶、顶叶和颞叶复合体。另一个优势是，猕猴的大脑是无脑的，含有大量的复合体，在患有大脑皮质球蛋白的猕猴中，这些复合体更难接触到大脑表面，这些特征允许直接接触到大脑表面下的大多数皮质区域。该系统提供了在时间上以亚毫秒级、在空间上以毫米级的高分辨率研究全球大脑皮层信息处理的机会。
Electrocorticography (ECoG) allows the monitoring of electrical field potentials from the cerebral cortex with high spatiotemporal resolution. Recent development of thin, flexible ECoG electrodes has enabled conduction of stable recordings of large-scale cortical activity. We have developed a whole-cortical ECoG array for the common marmoset. The array continuously covers almost the entire lateral surface of cortical hemisphere, from the occipital pole to the temporal and frontal poles, and it captures whole-cortical neural activity in one shot. This protocol describes a chronic implantation procedure of the array in the epidural space of the marmoset brain. Marmosets have two advantages regarding ECoG recordings, one being the homologous organization of anatomical structures in humans and macaques, including frontal, parietal, and temporal complexes. The other advantage is that the marmoset brain is lissencephalic and contains a large number of complexes, which are more difficult to access in macaques with ECoG, that are exposed to the brain surface.These features allow direct access to most cortical areas beneath the surface of the brain. This system provides an opportunity to investigate global cortical information processing with high resolutions at a sub-millisecond order in time and millimeter order in space.