Epidural focal brain cooling abolishes neocortical seizures in cats and non-human primates
Epidural focal brain cooling abolishes neocortical seizures in cats and non-human primates
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DOI：
10.1016/j.neures.2017.04.007
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发表时间：
2017-09-01
影响因子：
2.9
通讯作者：
Suzuki, Michiyasu
中科院分区：
文献类型：
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作者：
Inoue, Takao;Fujii, Masami;Suzuki, Michiyasu
局灶性脑冷却（FBC）正在难治性癫痫（IE）（包括癫痫持续状态（SE））的临床前试验中进行研究。这种方法已经在啮齿动物中作为癫痫疾病的可能治疗方法进行了研究，但需要更多来自大型动物研究的证据。为了提供FBC是IE安全有效治疗的证据，我们研究了使用钛冷却板的FBC是否可以减少或终止局灶性新皮质癫痫发作，而不会对脑组织产生显著影响。两只猫和两只猕猴被长期植入硬膜外FBC装置的躯体感觉和运动皮层。青霉素G通过颅内插管输送以诱导局部癫痫发作。在4只动物中的3只中，使用植入中期的冷却装置进行重复FBC（每周至少两次，每次30分钟;共3个月）。动物在给予青霉素G后表现出癫痫发作伴重复癫痫样放电（ED），这些放电在低于20 ℃的冷却下减少，无不良组织学影响。本研究结果提示，硬膜外FBC是IE和SE的一种安全有效的潜在治疗方法。(C)2017 Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Focal brain cooling (FBC) is under investigation in preclinical trials of intractable epilepsy (IE), including status epilepticus (SE). This method has been studied in rodents as a possible treatment for epileptic disorders, but more evidence from large animal studies is required. To provide evidence that FBC is a safe and effective therapy for IE, we investigated if FBC using a titanium cooling plate can reduce or terminate focal neocortical seizures without having a significant impact on brain tissue. Two cats and two macaque monkeys were chronically implanted with an epidural FBC device over the somatosensory and motor cortex. Penicillin G was delivered via the intracranial cannula for induction of local seizures. Repetitive FBC was performed using a cooling device implanted for a medium-term period (FBC for 30 min at least twice every week; 3 months total) in three of the four animals. The animals exhibited seizures with repetitive epileptiform discharges (EDs) after administration of penicillin G, and these discharges decreased at less than 20 degrees C cooling with no adverse histological effects. The results of this study suggest that epidural FBC is a safe and effective potential treatment for IE and SE. (C) 2017 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights reserved.