Sucrose phosphate synthase genes in plants belong to three different families
Sucrose phosphate synthase genes in plants belong to three different families
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DOI：
10.1007/s00239-001-0047-4
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发表时间：
2002-03-01
影响因子：
3.9
通讯作者：
MacRae, EA
中科院分区：
文献类型：
--
作者：
Langenkämper, G;Fung, RWM;MacRae, EA
我们目前的系统发育分析表明，有三个家庭的蔗糖磷酸合成酶（SPS）基因存在于高等植物。检查了两个数据集，一个由全长蛋白组成，第二个较大的数据集覆盖了高度保守的区域，包括14-3-3结合区和UDPGlu活性位点。对两个数据集的分析显示，已知基因被很好地分离为三个家族，命名为A、B和C。拟南芥的基因组序列包括每个家族中的一个成员：5号染色体上的两个基因属于家族A，1号染色体上的一个基因属于家族B，4号染色体上的一个基因属于家族C。三个柑橘基因中的每一个都属于三个家族中的一个。根据系统发育分析，这四个拟南芥基因的内含子/外显子组织不同，来自不同物种的同一家族的成员具有相似的SPS基因的基因组组织。拟南芥5号染色体上的两个A家族基因似乎是由于最近的重复。对已发表的文献和EST的分析表明，虽然B家族成员似乎不在根中表达，但家族的功能分化并不明显。从两种猕猴桃中克隆了B家族基因，Southern分析表明存在一个单一的基因家族，这与猕猴桃中家族A的多个成员形成对比。迄今为止，只有两个家族C基因被报道。
We present phylogenetic analyses to demonstrate that there are three families of sucrose phosphate synthase (SPS) genes present in higher plants. Two data sets were examined, one consisting of full-length proteins and a second larger set that covered a highly conserved region including the 14-3-3 binding region and the UDPGlu active site. Analysis of both datasets showed a well supported separation of known genes into three families, designated A, B, and C. The genomic sequences of Arabidopsis thaliana include a member in each family: two genes on chromosome 5 belong to Family A, one gene on chromosome 1 to Family B, and one gene on chromosome 4 to Family C. Each of three Citrus genes belong to one of the three families. Intron/exon organization of the four Arabidopsis genes differed according to phylogenetic analysis, with members of the same family from different species having similar genomic organization of their SPS genes. The two Family A genes on Arabidopsis chromosome 5 appear to be due to a recent duplication. Analysis of published literature and ESTs indicated that functional differentiation of the families was not obvious, although B family members appear not to be expressed in roots. B family genes were cloned from two Actinidia species and southern analysis indicated the presence of a single gene family, which contrasts to the multiple members of Family A in Actinidia. Only two family C genes have been reported to date.