Identification of Anaerobic Bacteria by Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry with On-Plate Formic Acid Preparation
Identification of Anaerobic Bacteria by Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry with On-Plate Formic Acid Preparation
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DOI：
10.1128/jcm.02420-12
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发表时间：
2013-03-01
影响因子：
9.4
通讯作者：
Patel, Robin
中科院分区：
文献类型：
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作者：
Schmitt, Bryan H.;Cunningham, Scott A.;Patel, Robin
使用表型方法鉴定厌氧菌通常很耗时; 16 S rRNA基因测序等方法成本高，可能不易获得。我们使用Bruker MALDI Biotyper（Bruker Daltonics，Billerica，MA）基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统（具有用户补充的数据库和基于甲酸的板上制备方法）评估了253株临床厌氧菌分离株，并将结果与使用生化检测或16 S rRNA基因测序的常规鉴定结果进行了比较。采用生物学家推荐的评分临界值，分别将179株（70.8%）和232株（91.7%）分离株正确鉴定为种和属水平。MALDI-TOF MS提供了一种快速、廉价的鉴定厌氧菌的方法。
Identification of anaerobic bacteria using phenotypic methods is often time-consuming; methods such as 16S rRNA gene sequencing are costly and may not be readily available. We evaluated 253 clinical isolates of anaerobic bacteria using the Bruker MALDI Biotyper (Bruker Daltonics, Billerica, MA) matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system with a user-supplemented database and an on-plate formic acid-based preparation method and compared results to those of conventional identification using biochemical testing or 16S rRNA gene sequencing. A total of 179 (70.8%) and 232 (91.7%) isolates were correctly identified to the species and genus levels, respectively, using manufacturer-recommended score cutoffs. MALDI-TOF MS offers a rapid, inexpensive method for identification of anaerobic bacteria.