Isometric contraction force measurement of hiPSC-CMs on a movable plate with a feedback-controlled MEMS cantilever probe
Isometric contraction force measurement of hiPSC-CMs on a movable plate with a feedback-controlled MEMS cantilever probe
复制标题
DOI：
10.1088/1361-6501/ac15dd
复制
发表时间：
2021-11-01
影响因子：
2.4
通讯作者：
Shimoyama, Isao
中科院分区：
文献类型：
--
作者：
Matsudaira, Kenei;Takahashi, Hidetoshi;Shimoyama, Isao
我们提出了一种带反馈系统的测量方法来评估动态力学环境下人iPS细胞来源的心肌细胞(hiPSC-CMS)的收缩力量。测量HiPSC-CM的收缩力量对于再生医学很重要；然而，当细胞受到类似于心脏的动态负荷时，传统方法无法评估这些力量。所提出的测量系统由连接在反馈控制压电台上的微机械压阻悬臂梁和用于培养细胞的微机械活动平板组成。通过反馈控制的压电式工作台实现了高采样率(2 KHz)和单元长度的实时控制，而收缩压力则由悬臂梁测量。我们评估了HiPSC-CMS在等长收缩和等张收缩条件下的收缩力。由于等长收缩的反馈控制加载，细胞收缩被控制在200 nm以下。在无反馈控制的情况下测得3.9mU的助张缩力，在反馈控制负荷下测得等长收缩的缩力为6.0mN。结果表明，该方法导致了对HiPSC-CM心动周期的工作循环评估。
We propose a measurement method with a feedback system to evaluate the contraction forces of human iPS cell-derived cardiomyocytes (hiPSC-CMs) in a dynamic mechanical environment. The measurement of the hiPSC-CM contraction forces is important for regenerative medicine; however, conventional methods are not able to evaluate these forces when the cells are subjected to a dynamic load similar to that from the heart. The proposed measurement system is composed of a micromachined piezoresistive cantilever attached to a feedback-controlled piezo stage and a micromachined movable plate where cells are cultured. A high sampling rate (2 kHz) and real-time control of the cell length were realized via the feedback-controlled piezo stage, while the contraction forces were measured by the cantilever. We evaluated the contraction forces of hiPSC-CMs in conditions of isometric and auxotonic contractions. Due to feedback-controlled loading for isometric contraction, the cell shrinkage was controlled to be less than 200 nm. Auxotonic contraction forces of 3.9 mu N were measured without feedback control, while the contraction forces of isometric contraction were 6.0 mu N with feedback-controlled loading. The results showed that the method leads to a work-loop evaluation of the hiPSC-CM cardiac cycle.