Induction of toxic granulation in neutrophils by granulocyte colony‐stimulating factor
Induction of toxic granulation in neutrophils by granulocyte colony‐stimulating factor
复制标题
粒细胞集落刺激因子诱导中性粒细胞有毒颗粒形成
DOI：
--
复制
发表时间：
2002
影响因子：
3.1
通讯作者：
Yoshinori I Watani
中科院分区：
文献类型：
--
作者：
O. Kabutomori;Y. Kanakura;Yoshinori I Watani
To the Editor: Toxic granulation neutrophils (TGNs), which have prominent azurophilic cytoplasmic granules in blood smears stained by the Wright or May– Grünwald–Giemsa technique, appear in blood when inflammation occurs. Toxic granulation in neutrophils is an increase in acid mucosubstance in azurophilic granules (1). There is a positive correlation between the levels of C-reactive protein and the percentages of TGNs in blood samples from patients with inflammatory diseases (2), and so TGNs are one of the important inflammation makers (3). Inflammatory response is the mechanism by which the body defends against infection and repairs tissue damage, and the serum concentration of granulocyte colony-stimulating factor (G-CSF) and interleukin-6 increases in inflammation. It was reported that G-CSF induced TGN formation, but the concentration of G-CSF used in the study (10–100 lg mL) (4) was markedly high, as compared with that in blood from patients with infection (30–3199 pg mL) (5). In this study therefore we examined the effect of physiological levels of G-CSF on TGN formation. Neutrophils changed to TGN-like cells in response to 1000 pg mL of G-CSF added in peripheral blood from a healthy subject. Both the size and the number of azurophilic granules in those TGN-like cells were smaller [Fig. 1A(a)] than those in TGNs in peripheral blood from a patient with inflammatory disease [Fig. 1A(b)]. The formation of TGNlike cells was examined 30, 60, 90, and 120 min after the addition of 100, 500, and 1000 pg mL of G-CSF to peripheral blood samples from six healthy subjects. TGNs or TGN-like cells were counted on blood films stained by the May– Grünwald–Giemsa technique. Two hundred mature neutrophils were examined (Magnification ·1000), and the results were expressed as the percentage of TGN-like cells in mature neutrophils. The percentages of TGN-like cells increased in response to G-CSF in both a doseand timedependent manner (Fig. 1B). We observed increases of TGNs in peripheral blood from three patients during treatment with recombinant human G-CSF to support myelopoiesis under cancer chemotherapy (data not shown). These data indicate that G-CSF induces toxic granules in neutrophils. The significance of toxic granules induced by G-CSF in inflammation is still
pH 对中性粒细胞吞噬溶酶体成分杀死金黄色葡萄球菌和大肠杆菌的影响。
影响因子：
3
作者：
Styrt,B;Klempner,MS
通讯作者：
Klempner,MS