Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
Dynamics of homologous chromosome pairing during meiotic prophase in fission yeast
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DOI：
10.1016/s1534-5807(04)00059-0
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发表时间：
2004-03-01
影响因子：
11.8
通讯作者：
Hiraoka, Y
中科院分区：
文献类型：
--
作者：
Ding, DQ;Yamamoto, A;Hiraoka, Y
在减数分裂过程中，同源染色体配对对于同源重组和正确的染色体分离是重要的。有人认为，在分裂前期，端粒聚集、核振荡和重组促进了分裂酵母中同源染色体的配对。在这里，我们通过直接观察分裂酵母活细胞中染色体基因座的动态，研究了这些染色体事件对同源染色体配对的贡献。在减数分裂前期的时间过程中，同源基因座呈现出一个动态的结合和解离过程。缺乏核振荡降低了染色体着丝粒和臂区域的关联频率。缺乏端粒聚集或重组降低了ARM区域的关联频率，但在着丝粒区域并不显著。我们的结果表明，同源染色体通过端粒束染色体的振荡在空间上对齐，并通过染色体臂区域的重组物理连接；这种重组不是同源着丝粒联合所必需的。
Pairing of homologous chromosomes is important for homologous recombination and correct chromosome segregation during meiosis. It has been proposed that telomere clustering, nuclear oscillation, and recombination during meiotic prophase facilitate homologous chromosome pairing in fission yeast. Here we examined the contributions of these chromosomal events to homologous chromosome pairing, by directly observing the dynamics of chromosomal loci in living cells of fission yeast. Homologous loci exhibited a dynamic process of association and dissociation during the time course of meiotic prophase. Lack of nuclear oscillation reduced association frequency for both centromeric and arm regions of the chromosome. Lack of telomere clustering or recombination reduced association frequency at arm regions, but not significantly at centromeric regions. Our results indicate that homologous chromosomes are spatially aligned by oscillation of telomere-bundled chromosomes and physically linked by recombination at chromosome arm regions; this recombination is not required for association of homologous centromeres.