Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook
Contemporary Advancement of Cholinium-Based Ionic Liquids for Protein Stability and Long-Term Storage: Past, Present, and Future Outlook
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DOI：
10.1021/acssuschemeng.1c08595
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发表时间：
2022-04-11
影响因子：
8.4
通讯作者：
Venkatesu, Pannuru
中科院分区：
文献类型：
--
作者：
Sindhu, Anamika;Kumar, Sumit;Venkatesu, Pannuru
在过去的二十年里，离子液体(ILS)被公认为具有潜在吸引力的“绿色”和“设计型”溶剂，因为它具有更广阔的化学空间和各种离子的可调性，可以很好地调节它们的物理化学性质，同时能够实现特定任务的优化。它们在从酶反应到蛋白质保存等领域都有广泛的应用，主要集中在开发各种类型的ILS及其在体外提供蛋白质稳定性方面的应用。在不同的ILS家族中，有些ILS具有类似于有机溶剂的严重限制；由于它们不能生物降解，因此会造成环境毒性。换句话说，一些ILsimpart会对生物分子施加压力，并最终使蛋白质变性。在这方面，对生物相容性ILS的需求已经显现出来，基于胆碱的ILS(ChN ILS)已经被证明是支持生物分子结构的最有前途的ILS。中国ILS家族只是在最近几年才有所增长。尽管有大量的研究，但在CHIL和生物分子领域的更全面的研究仍然需要加速。在此，我们回顾了在蛋白质和基于酶的应用中应用CHN ILS的策略和目前的进展，同时考虑到所有关键的过去和现在的研究成果。此外，我们阐明了在CHN ILS存在下提高蛋白质的酶活性、结构稳定性和长期储存的各种方法的概述。我们相信，这些见解将有助于在生物技术的不同领域设计基于ILS的各种工艺，ILS将作为蛋白质稳定和酶反应的新溶剂，保持其在工业和生物医学方面的应用。种类繁多的生物基CHN ILS为正确选择酶的长期储存提供了最新的进展和发展的希望
In the past two decades, ionic liquids (ILs) have been acknowledged aspotentially attractive"green"and"designer"solvents since their broader chemical space andthe tunable nature of the diverse ions canfinely modulate their physiochemical propertieswhile enabling task-specific optimization. They have found numerous applications infieldsranging from enzymatic reactions to protein preservation, which focused on the developmentof various types of ILs and their application for providing protein stabilityin vitro. Amongvarious families of ILs, some of them have serious limitations similar to organic solvents; theycause environmental toxicity as they are non-biodegradable. In other words, some of the ILsimpart stress to biomolecules and ultimately denature the protein. In this regard, the need forbiocompatible ILs has come to the light and cholinium-based ILs (Chn ILs) have provedthemselves as the most promising ILs to support the structure of biomolecules. The family ofChn ILs has only taken growth in recent years. Despite the numerous studies, moreexhaustive research in thefield of Chn ILs and biomolecules still needs acceleration. Herein,we review the strategies and current progress on Chn ILs for protein and enzyme-basedapplications keeping in mind all crucial past and present research outcomes. Furthermore, we elucidate an overview of the variousways to enhance enzymatic activity, structural stability, and long-term storage of proteins in the presence of Chn ILs. We believethese insights will be fruitful for designing various processes based on ILs in the diversefield of biotechnology and ILs will serve asnovel solvents for protein stability and enzymatic reactions, maintaining their utility in industrial and biomedical based applications.The huge varieties of biobased Chn ILs hold the promise of recent advances and developments for the correct selection of long-termstorage of enzymes