Sex-dependent and organ-specific toxicity of silver nanoparticles in livers and intestines of adult zebrafish
Sex-dependent and organ-specific toxicity of silver nanoparticles in livers and intestines of adult zebrafish
复制标题
银纳米颗粒对成年斑马鱼肝脏和肠道的性别依赖性和器官特异性毒性
DOI：
10.1016/j.chemosphere.2020.126172
复制
发表时间：
2020-06-01
期刊：
影响因子：
8.8
通讯作者：
Fang, Tao
中科院分区：
文献类型：
--
作者：
Bao, Shaopan;Tang, Wei;Fang, Tao
银纳米颗粒（AgNPs）的制造越来越多，因此在水生系统中检测到的越来越多。然而，仍然有一些被忽视的因素（例如，生物性别）在纳米毒理学评估领域。在这项研究中，为了探索性别在纳米毒性中的作用，成年雄性和雌性斑马鱼暴露于100 μ g/L的两种未涂覆的商业银纳米颗粒，主要尺寸为20 nm和80 nm，持续2周，之后使用一套生物标志物评估银纳米颗粒对雄性和雌性斑马鱼肠道和肝脏的影响。结果表明，20-nm和80-nm AgNPs处理组雄性斑马鱼肠道Na/KATP酶活性和超氧化物歧化酶活性差异显著（p < 0.05），表明20-nm AgNPs比80-nm AgNPs显示出更高的毒性。此外，我们注意到，所使用的AgNPs诱导的生长指标，氧化/抗氧化状态，神经信号和肝脏脂质代谢的性别依赖性的影响，与雄性斑马鱼更敏感的AgNPs比女性。此外，所测试的AgNPs对肠的损害比肝脏严重得多，如肠中Na/KATP酶和抗氧化系统的破坏而非肝脏所证明的。这些结果表明，长期暴露于AgNPs可能会诱导成年斑马鱼的大小相关，性别依赖性和器官特异性的毒性，从而可能会显着扩展我们的理解AgNPs在水环境中的毒性效应。(C)2020爱思唯尔有限公司保留所有权利。
Silver nanoparticles (AgNPs) have been increasingly manufactured and thus are increasingly detected in aquatic systems. However, there are still some overlooked factors (e.g., organism sex) in the field of nanotoxicological assessment. In this study, to explore the role of sex in nanotoxicity, adult male and female zebrafish were exposed to 100 mu g/L of two uncoated commercial AgNPs with primary sizes 20 nm and 80 nm for 2 weeks, after which the impacts of AgNPs on intestines and livers of both male and female zebrafish were assessed using a suite of biomarkers. Results demonstrated that the intestinal Na/KATPase activity as well as the superoxide dismutase activity in male zebrafish differed significantly between 20-nm AgNPs and 80-nm AgNPs treatments (p < 0.05), indicating 20-nm AgNPs showing higher toxicity to zebrafish than the 80-nm AgNPs. Also, we noted that the used AgNPs induced sex-dependent effects on growth indices, oxidative/anti-oxidative status, neural signaling and hepatic lipid metabolism, with the male zebrafish being more sensitive to AgNPs than the females. Further, the tested AgNPs impaired the intestine much more seriously than the liver, as evidenced by the disruptions of Na/KATPase and antioxidant system in intestine but not in liver. These findings imply that prolonged exposure to AgNPs might induce size-related, sex-dependent, and organ-specific toxicity to adult zebrafish, thereby may significantly extend our understanding of the toxic effects of AgNPs in aquatic environment. (C) 2020 Elsevier Ltd. All rights reserved.