Fibroblast growth factor 2 enhances early stages of in vitro endothelial repair by microfilament bundle reorganization and cell elongation.
Fibroblast growth factor 2 enhances early stages of in vitro endothelial repair by microfilament bundle reorganization and cell elongation.
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成纤维细胞生长因子 2 通过微丝束重组和细胞伸长增强体外内皮修复的早期阶段。
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Gotlieb
中科院分区：
文献类型：
--
作者：
D. I. Wang;A. Gotlieb
随着内皮细胞在8小时内沿创面边缘从静止细胞转变为迁移细胞，肌动蛋白微丝经历了明确的顺序变化，其特征是微丝相对于创面边缘的初始随机分布，然后平行，然后垂直。后者与随后的细胞迁移有关。我们验证了一个假设，即成纤维细胞生长因子2 （FGF-2）可以增强伤口修复的早期阶段，甚至在迁移之前，并且FGF-2对伤口修复的增强与内皮肌动蛋白细胞骨架的变化有关。在体外双侧创面模型中，在损伤时添加FGF-2可在8小时内增强创面愈合程度。在损伤后4和8小时，FGF-2处理可显著延长创面边缘的细胞长度。当增加中和性FGF-2抗体浓度时，伤口愈合的程度在8小时内下降，并与伤口边缘细胞长度的减少有关。用罗丹明法定位肌动蛋白微丝，用激光共聚焦显微镜观察。在受伤后4小时，与未处理的细胞相比，FGF-2处理显著增加了沿伤口表达垂直微丝的细胞，这表明平行向垂直微丝分布的转变更快。因此，由于早期出现垂直微丝和沿伤口边缘的细胞延长，FGF-2通过促进伤口闭合来影响迁移之前的伤口修复的早期阶段。
As endothelial cells convert from quiescent to migrating cells over 8 h along a wound edge, actin microfilaments undergo well-defined sequential changes characterized by an initial random distribution followed by a parallel and then a perpendicular orientation of microfilaments with respect to the wound edge. The latter is associated with subsequent cell migration. We tested the hypothesis that fibroblast growth factor 2 (FGF-2) can enhance the very early stages of wound repair even prior to migration and that FGF-2 enhancement of wound repair is associated with changes in the endothelial actin cytoskeleton. Using an in vitro two-sided wound model, the addition of FGF-2 at the time of wounding enhanced the extent of wound closure over 8 h. Treatment with FGF-2 was associated with significantly longer cells along the wound edge at 4 and 8 h after wounding. When treated with increasing concentrations of neutralizing FGF-2 antibody, the extent of wound closure decreased over 8 h and was associated with a decrease in cell length along the wound edge. Actin microfilaments were localized using rhodamine phalloidin and viewed using laser confocal microscopy. At 4 h after wounding, FGF-2 treatment was associated with significantly more cells along the wound expressing perpendicular microfilaments compared to untreated cells, which suggested a more rapid transition of parallel to perpendicular microfilament distribution. Thus, FGF-2 affects the very early stages of wound repair prior to migration by enhancing wound closure due to the early appearance of perpendicular microfilaments and lengthening of cells along the wound edge.
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动脉损伤后细胞增殖的动力学。
DOI：
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发表时间：
1986
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Clowes,AW;Clowes,MM;Reidy,MA
通讯作者：
Reidy,MA
碱性成纤维细胞生长因子刺激的内皮细胞运动是由调节磷脂酶 A2 活性的百日咳毒素敏感途径介导的。
DOI：
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发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Sa,G;Fox,PL
通讯作者：
Fox,PL
影响因子：
15.9
作者：
Murugesan, G;Fox, PL
通讯作者：
Fox, PL
影响因子：
37.8
作者：
Schaff, Mathieu;Tang, ChaoJun;Mangin, Pierre H.
通讯作者：
Mangin, Pierre H.
影响因子：
15.9
作者：
BROGI, E;WINKLES, JA;LIBBY, P
通讯作者：
LIBBY, P