Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging
Cell membrane conformation at vertical nanowire array interface revealed by fluorescence imaging
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DOI：
10.1088/0957-4484/23/41/415102
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发表时间：
2012-10-19
期刊：
影响因子：
3.5
通讯作者：
Martinez, Karen L.
中科院分区：
文献类型：
--
作者：
Berthing, Trine;Bonde, Sara;Martinez, Karen L.
纳米线垂直阵列在活细胞中的生物传感应用所提供的前景取决于单个纳米线对细胞内部的访问。最近的研究结果表明，电通路和分子传递的直接访问并不总是获得。在这里，我们提出了一个通用的方法来直接可视化的活细胞的膜构象与纳米线阵列，与单纳米线的分辨率。该方法结合了共聚焦z-堆栈成像与优化的细胞膜标记策略，该策略应用于与2-11 μ m长和3-7 μ m间隔的纳米线连接的HEK 293细胞，纳米线具有各种表面涂层（裸露的、氨基硅烷涂覆的或聚乙烯亚胺涂覆的砷化铟）。我们证明，对于所有常用的纳米线长度，间距和表面涂层，纳米线通常保持封闭在膜室中，从而不与细胞内部直接接触。
The perspectives offered by vertical arrays of nanowires for biosensing applications in living cells depend on the access of individual nanowires to the cell interior. Recent results on electrical access and molecular delivery suggest that direct access is not always obtained. Here, we present a generic approach to directly visualize the membrane conformation of living cells interfaced with nanowire arrays, with single nanowire resolution. The method combines confocal z-stack imaging with an optimized cell membrane labelling strategy which was applied to HEK293 cells interfaced with 2-11 mu m long and 3-7 mu m spaced nanowires with various surface coatings (bare, aminosilane-coated or polyethyleneimine-coated indium arsenide). We demonstrate that, for all commonly used nanowire lengths, spacings and surface coatings, nanowires generally remain enclosed in a membrane compartment, and are thereby not in direct contact with the cell interior.