The vitamin E derivative garcinoic acid from Garcinia kola nut seeds attenuates the inflammatory response
The vitamin E derivative garcinoic acid from Garcinia kola nut seeds attenuates the inflammatory response
复制标题
DOI：
10.1016/j.redox.2019.101166
复制
发表时间：
2019-06-01
期刊：
影响因子：
11.4
通讯作者：
Lorkowski, Stefan
中科院分区：
文献类型：
--
作者：
Wallert, Maria;Bauer, Julia;Lorkowski, Stefan
在非洲民族医学中，植物藤黄用于治疗各种氧化和炎症相关疾病，但其生物活性化合物尚未得到很好的表征。Garcinoic acid（GA）是从藤黄果中分离得到的为数不多的植物化学物质之一，我们研究了藤黄果种子的甲醇提取物（NE）和纯化的GA的抗炎潜力，GA是这些种子中的主要植物化学物质，脂多糖（LPS）激活的小鼠RAW 264.7巨噬细胞及其在高脂饮食喂养ApoE（-/-）中的抗动脉粥样硬化潜力本研究概述了一种从藤黄种子中提取和纯化GA的优化方法，其产量增加，纯度> 99%。我们发现，LPS诱导的iNos和Cox 2表达的上调，以及各自的信号分子一氧化氮和前列腺素的形成，显着减少了NE和GA。此外，GA治疗小鼠通过减弱硝基酪氨酸化减少斑块内炎症。此外，调制的淋巴细胞亚群在血液和脾脏中已被检测到，显示GA的免疫调节特性。我们的研究提供了分子洞察藤黄的抗炎活性，并揭示了GA作为有前途的天然铅的发展多靶点药物治疗炎症驱动的疾病。
The plant Garcinia kola is used in African ethno-medicine to treat various oxidation- and inflammation-related diseases but its bioactive compounds are not well characterized. Garcinoic acid (GA) is one of the few phyto-chemicals that have been isolated from Garcinia kola.We investigated the anti-inflammatory potential of the methanol extract of Garcinia kola seeds (NE) and purified GA, as a major phytochemical in these seeds, in lipopolysaccharide (LPS)-activated mouse RAW264.7 macrophages and its anti-atherosclerotic potential in high fat diet fed ApoE(-/-) mice.This study outlines an optimized procedure for the extraction and purification of GA from Garcinia kola seeds with an increased yield and a purity of > 99%. We found that LPS-induced upregulation of iNos and Cox2 expression, and the formation of the respective signaling molecules nitric oxide and prostanoids, were significantly diminished by both the NE and GA. In addition, GA treatment in mice decreased intra-plaque inflammation by attenuating nitrotyrosinylation. Further, modulation of lymphocyte sub-populations in blood and spleen have been detected, showing immune regulative properties of GA.Our study provides molecular insights into the anti-inflammatory activities of Garcinia kola and reveals GA as promising natural lead for the development of multi-target drugs to treat inflammation-driven diseases.