Enhancing precision in time-domain fluorescence lifetime imaging
Enhancing precision in time-domain fluorescence lifetime imaging
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DOI：
10.1117/1.3494566
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发表时间：
2010-09-01
影响因子：
3.5
通讯作者：
Mycek, Mary-Ann
中科院分区：
文献类型：
--
作者：
Chang, Ching-Wei;Mycek, Mary-Ann
在荧光寿命成像的生物学应用中，来自样品的低信号可能是一个挑战，导致寿命精度差。我们展示了如何在时域荧光寿命成像显微镜（FLIM）中使用最佳信号门控（一种应用于寿命图像的时间维度的方法）和新颖的全变差去噪模型（一种应用于寿命图像的空间维度的方法）来提高寿命精度。在时间门控FLIM中，在低光情况下观察到显着的四倍精度提高。这种方法可以用来改善胶片成像数据，同时最大限度地减少样品曝光和提高成像速度。(C) 2010光学仪器工程师学会。(DOI: 10.1117/1.3494566)
In biological applications of fluorescence lifetime imaging, low signals from samples can be a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a method applied to the temporal dimension of a lifetime image) and novel total variation denoising models (a method applied to the spatial dimension of a lifetime image) can be used in time-domain fluorescence lifetime imaging microscopy (FLIM) to improve lifetime precision. In time-gated FLIM, notable fourfold precision improvements were observed in a low-light example. This approach can be employed to improve FLIM data while minimizing sample light exposure and increasing imaging speed. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3494566]