Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease
Effect of Resistant Starch on the Gut Microbiota and Its Metabolites in Patients with Coronary Artery Disease
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DOI：
10.5551/jat.47415
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发表时间：
2019-08
影响因子：
4.4
通讯作者：
N. Yoshida;K. Sasaki;D. Sasaki;T. Yamashita;Hajime Fukuda;Tomohiro Hayashi;Tokiko Tabata;R. Osawa;K. Hirata;A. Kondo
中科院分区：
文献类型：
--
作者：
N. Yoshida;K. Sasaki;D. Sasaki;T. Yamashita;Hajime Fukuda;Tomohiro Hayashi;Tokiko Tabata;R. Osawa;K. Hirata;A. Kondo
目的：普通拟杆菌（Bacteroides vulgatus）和多氏杆菌（b.d orei）对动脉粥样硬化具有保护作用，表明肠道微生物群中这些物种的扩大可能有助于冠心病（CAD）患者。本研究旨在通过单批发酵系统研究抗性淀粉（RS）对CAD患者和非CAD个体粪便样本培养中肠道微生物群及其代谢物的影响。方法：将11例CAD患者和10例非CAD患者的粪便样本在神户大学人体肠道微生物群模型（KUHIMM）中进行有或无RS发酵30 h。采用16S核糖体核糖核酸（rRNA）基因测序和定量聚合酶链反应分析肠道菌群及普通双歧杆菌和多歧杆菌的丰度。采用高效液相色谱法对短链脂肪酸进行分析。结果：在KUHIMM中发酵30 h后，对CAD患者原始粪便样本进行肠道微生物分析，结果显示CAD患者原始粪便样本中普通芽孢杆菌和多雷芽孢杆菌水平明显降低。虽然RS显著增加了CAD粪便样本培养中普通芽孢杆菌和多芽孢杆菌的绝对数量和丁酸盐水平，但每个患者的数量不同。结论：RS对CAD和非CAD粪便样本培养的KUHIMM肠道微生物群及其代谢物的影响存在差异。KUHIMM可能有助于RS对肠道微生物群及其代谢物影响的临床前评估。
Aim: Bacteroides vulgatus and B. dorei have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system. Methods: Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of B. vulgatus and B. dorei were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography. Results: Gut microbial analysis showed significantly lower levels of B. vulgatus and B. dorei in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of B. vulgatus and B. dorei, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient. Conclusions: The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.