ATR-FTIR spectroscopy and chemometric analysis applied to discrimination of landrace maize flours produced in southern Brazil
ATR-FTIR spectroscopy and chemometric analysis applied to discrimination of landrace maize flours produced in southern Brazil
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DOI：
10.1111/j.1365-2621.2010.02313.x
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Maraschin, Marcelo
中科院分区：
文献类型：
--
作者：
Kuhnen, Shirley;Ogliari, Juliana Bernardi;Maraschin, Marcelo
P>这项工作旨在通过使用衰减全反射傅里叶变换红外 (ATR-FTIR) 光谱和化学计量学（主成分分析 - PCA）来区分来自巴西南部的 26 种玉米地方品种的面粉。 PCA 应用于 3-600（整个光谱）和 1650-1500 cm-1（蛋白质指纹区域）光谱窗口中的 FTIR 光谱，可以清楚地区分 Amarelao 地方品种。蛋白质的定量和半定性分析显示各组分之间存在很大差异，主要是谷醇溶蛋白（13.47-28.43 g Kg-1）和谷蛋白（5.57-30.98 g Kg-1）含量。 Pixurum 6、Pixurum 5 和 MPA1 地方品种因其白蛋白、球蛋白和谷蛋白含量而具有卓越的营养价值。碳水化合物指纹区域（1200-950 和 1065-950 cm-1）的光谱数据集的 PCA（还包括直链淀粉和支链淀粉的商业标准品）能够分离 Moroti 基因型，该基因型与支链淀粉标准品分组。因此，ATR-FTIR 和 PCA 被证明是快速筛选和区分具有不同化学成分的玉米的有用工具。
P>This work aims at discriminating flours of 26 maize landraces from southern Brazil, by using the Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy and chemometrics (principal components analysis - PCA). PCA applied to the FTIR spectra in the 3-600 (whole spectrum) and 1650-1500 cm-1 (fingerprint region of proteins) spectral windows clearly discriminated the Amarelao landrace. Quantitative and semi-qualitative analysis of proteins showed a wide range among the fractions, mainly of prolamine (13.47-28.43 g Kg-1) and glutelin (5.57-30.98 g Kg-1) contents. Pixurum 6, Pixurum 5, and MPA1 landraces are of superior nutritional value for their albumin, globulin, and glutelin contents. PCA of the spectral dataset in the fingerprint region to carbohydrates (1200-950 and 1065-950 cm-1) also including commercial standards of amylose and amylopectin was able in separating the Moroti genotype, which grouped with the amylopectin standard. Thus, ATR-FTIR and PCA showed to be useful tools for the quick screening and discrimination of maize with distinct chemical composition.