Platelet transcriptome: The application of microarray analysis to platelets
Platelet transcriptome: The application of microarray analysis to platelets
复制标题
DOI：
10.1055/s-2004-833482
复制
发表时间：
2004-08-01
影响因子：
5.7
通讯作者：
Gnatenko, DV
中科院分区：
文献类型：
--
作者：
Bahou, WF;Gnatenko, DV
人类的血小板与血栓形成、炎症和伤口修复的调节密切相关。这些细胞保留了巨核细胞来源的细胞质mRNA和功能完整的蛋白质翻译能力，尽管对正常或病理的mRNA谱知之甚少。微阵列分析已经证明了血小板特有的清晰和可重现的分子特征。与在其他真核细胞中发现的相比，表达的转录本相对较少，很可能与这些无核细胞中的mRNA衰退有关。相反，一种互补的转录谱分析方法(基因表达序列分析[SAGE])表明，89%的标签代表线粒体(Mt)转录本(富含16S和12S核糖体RNA)，推测与没有核来源转录本的持久mt转录有关。非线粒体SAGE标签的丰度与微阵列分析确定的最丰富的转录本的相对表达平行，建立了两种血小板图谱技术的一致性。这些观察证实了转录分析作为一种工具用于识别可能调节正常和病理性血小板(和/或巨核细胞)功能的新的血小板基因的有效性。综述了血小板特异性微阵列在与血小板生成、心血管和脑血管疾病相关的科学和临床环境中的潜在应用。
Human blood platelets are intimately involved in the regulation of thrombosis, inflammation, and wound repair. These cells retain megakaryocyte-derived cytoplasmic mRNA and functionally intact protein translational capabilities, although very little is known about normal or pathological mRNA profiles. Microarray analysis has demonstrated a clear and reproducible molecular signature unique to platelets. There is a relative paucity of expressed transcripts compared with those found in other eukaryotic cells, most likely related to mRNA decay in these anucleate cells. In contrast, a complementary methodology for transcript profiling (serial analysis of gene expression [SAGE]) demonstrates that 89% of tags represent mitochondrial (mt) transcripts (enriched in 16S and 12S ribosomal RNAs), presumably related to persistent mt-transcription in the absence of nuclear-derived transcripts. The abundance of nonmitochondrial SAGE tags parallels relative expression for the most abundant transcripts as determined by microarray analysis, establishing the concordance of both techniques for platelet profiling. These observations establish the validity of transcript analysis as a tool for identifying novel platelet genes that may regulate normal and pathologic platelet (and/or megakaryocyte) functions. The potential application of platelet-specific microarrays in scientific and clinical settings related to platelet production, cardiovascular, and cerebrovascular diseases is reviewed.