STROMA OF HUMAN BENIGN PROSTATIC HYPERPLASIA - PREFERENTIAL TISSUE FOR ANDROGEN METABOLISM AND ESTROGEN BINDING
STROMA OF HUMAN BENIGN PROSTATIC HYPERPLASIA - PREFERENTIAL TISSUE FOR ANDROGEN METABOLISM AND ESTROGEN BINDING
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DOI：
10.1530/acta.0.0960422
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
VOIGT, KD
中科院分区：
文献类型：
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作者：
KRIEG, M;KLOTZL, G;VOIGT, KD
由于各种泌尿生殖器官间质-上皮相互作用是众所周知的，因此定量比较人类良性前列腺增生（BPH）上皮(E)和间质(S)中类固醇代谢和结合是有意义的。睾丸激素5. alpha。-还原酶活性采用薄层色谱法测定，雄激素结合位点和雌激素结合位点采用炭吸附法测定。C，使用甲基三烯诺酮（R1881）和雌二醇-17 - β。作为氚化配体，未标记的R1881和己烯雌酚作为各自的竞争对手。主要结果如下：利用酸性磷酸酶、羟脯氨酸等生化指标，发现E被S污染的平均比例为17%，S被E污染的平均比例为6%；在E和S中，NADPH酶反应的摩尔最优值在1 ~ 0.1 mM之间，几乎相同；5 α的视Km。-还原酶在两个组分中相同，平均值为0.15 μ m；最大速率是5。-还原酶活性（pmol 5. α）-减少代谢物。Mg蛋白-1 .cntdot。1 h-1)等于161。28 (SEM[平均值的标准误差]；n = 20), 66 .+-。4.6和148 .+-。BPH的S、E和全组织部分分别为6.6。2例正常前列腺的平均值分别为：88、53、73；雄激素结合位点均匀分布在E和S的细胞质中，而在42%的S中发现了可测量的雌激素结合位点，而在5%的E中发现了可测量的雌激素结合位点，高2.4倍。与BPH的E相比，S中的-还原酶活性是导致。与正常前列腺相比，BPH的整个组织部分的活性高2-2.5倍。根据初步的结合研究，雌激素可能在BPH的S部分中起重要作用。
Because of the well known stromal-epithelial interaction of various urogenital organs, it was of interest to compare quantitatively steroid metabolism and binding in epithelium (E) and stroma (S) of the human benign prostatic hyperplasia (BPH). Testosterone 5.alpha.-reductase activity was determined by TLC and androgen as well as estrogen binding sites by a charcoal adsorption technique after a steroid incubation period of 18 h at 0.degree. C, using methyltrienolone (R1881) and estradiol-17.beta. as tritiated ligands and unlabled R1881 and diethylstilbestrol as the respective competitors. The main results were as follows: using biochemical markers (acid phosphatase, hydroxyproline), an average 17% contamination of E by S and 6% of S by E was found; the molar optimum of NADPH for the enzyme reaction was nearly identical in E and S, ranging between 1 and 0.1 mM; the apparent Km of 5.alpha.-reductase was identical in both fractions, the mean being 0.15 .mu.M; the maximal rate of 5.alpha.-reductase activity (pmol 5.alpha.-reduced metabolites .cntdot. mg protein-1 .cntdot. 1 h-1) was 161 .+-. 28 (SEM [standard error of the mean]; n = 20), 66 .+-. 4.6 and 148 .+-. 6.6 in S, E and whole tissue fraction of BPH, respectively. In 2 normal prostates the means were: 88, 53 and 73, respectively; the androgen binding sites were evenly distributed between the cytosol of E and S, while measurable estrogen binding sites were found in 42% of the analyzed S but only in 5% of analyzed E. The 2.4 times higher 5.alpha.-reductase activity in S compared to E of the BPH is responsible for the .apprx. 2-2.5 times higher activity in the whole tissue fraction of BPH if compared with the normal prostate. Due to preliminary binding studies, estrogens might play an important role in the S fraction of BPH.