Distinct but redundant expression of the Frizzled Wnt receptor genes at signaling centers of the developing mouse brain
Distinct but redundant expression of the Frizzled Wnt receptor genes at signaling centers of the developing mouse brain
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DOI：
10.1016/j.neuroscience.2007.04.060
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发表时间：
2007-07-13
期刊：
影响因子：
3.3
通讯作者：
Prakash, N.
中科院分区：
文献类型：
--
作者：
Fischer, T.;Guimera, J.;Prakash, N.
脊椎动物中枢神经系统区域划分的建立是通过不同的神经上皮组织中心的活动来完成的。无翼/INT(WnT)家族分泌的糖蛋白等在这些中心的信号传递中起着至关重要的作用。例如，从中脑和后脑之间的交界处分泌的WNT1控制着这一大脑区域和相关神经元群体的发育。不同的令状从尾内侧大脑皮层分泌，皮质下摆，形成邻近的海马区。Wnt信号转导的第一步是将Wnt配体与其受体--七通跨膜Frizzled蛋白质结合。小鼠体内不同Frizzled型基因的失活揭示了这些受体之间广泛的功能冗余。为了区分不同的Frizzled型受体在Wnt信号转导中的可能参与，我们对小鼠胚胎发育关键阶段的10个已知的Frizzled型基因进行了详细的表达研究。我们的分析揭示了单个Frizzled型基因在发育中的小鼠胚胎的前神经管中高度动态而独特的表达模式。然而，在发育中的小鼠大脑的任何区域，至少有两个、最多六个Frizzled基因的时空重叠表达，也表明小鼠Frizzled型受体存在巨大的功能冗余。在未来对小鼠这些信号中心的Frizzled型受体功能进行分析时，必须考虑这种冗余性。(C)2007年IBRO。爱思唯尔有限公司出版。保留所有权利。
The establishment of the regional subdivisions of the vertebrate CNS is accomplished through the activity of different neuroepithelial organizing centers. The wingless/int (Wnt) family of secreted glycoproteins, among other factors, plays a crucial role in signaling from these centers. Wnt1 secreted from the boundary between the mid- and hindbrain, for instance, controls the development of this brain region and of associated neuronal populations. Different Writs secreted from the caudomedial pallium, the cortical hem, pattern the adjacent hippocampal field. The first step in Wnt signal transduction is binding of the Wnt ligand to its receptors, the seven-pass transmembrane Frizzled proteins. Inactivation of different Frizzled genes in mice have revealed an extensive functional redundancy between these receptors. In order to discriminate between a possible participation of different Frizzled receptors in the transduction of Wnt signals at the mid-/hindbrain boundary and the cortical hem, we have per-formed a detailed expression study of the 10 known murine Frizzled genes at crucial stages of mouse embryonic development. Our analysis reveals a highly dynamic yet distinct expression pattern of individual Frizzled genes in the anterior neural tube of the developing mouse embryo. The overlapping spatio-temporal expression of at least two and up to six Frizzled genes in any region of the developing mouse brain, however, also suggests a vast functional redundancy of the murine Frizzled receptors. This redundancy has to be taken into consideration for future analyses of Frizzled receptor function at these signaling centers in the mouse. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.