Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.
Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.
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人胸苷激酶基因启动子内包含的序列可以指导异源融合基因的细胞周期调节。
DOI：
10.1073/pnas.85.16.5894
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发表时间：
1988
影响因子：
11.1
通讯作者：
Lee,AS
中科院分区：
文献类型：
--
作者：
Kim,YK;Wells,S;Lau,YF;Lee,AS
最近的证据对人类胸苷激酶（TK）基因的转录调控提出了可能性，细胞周期调控序列可能位于其启动子。将TK基因5'端侧翼序列与细菌新霉素抗性基因（neo）编码区结合，构建了一个杂合基因。在转染到仓鼠成纤维细胞系K12中后，杂交基因表现出细胞周期依赖性表达。缺失分析表明，对细胞周期调控重要的区域在转录起始位点的-441到-63个核苷酸内。该区域（-441至-63）也赋予单纯疱疹病毒胸苷激酶（HSVtk）启动子细胞周期调节，该启动子不以细胞周期方式表达。我们的结论是，-441至-63序列内的人TK启动子是重要的细胞周期依赖性表达。
Recent evidence on the transcriptional regulation of the human thymidine kinase (TK) gene raises the possibility that cell-cycle regulatory sequences may be localized within its promoter. A hybrid gene that combines the TK 5' flanking sequence and the coding region of the bacterial neomycin-resistance gene (neo) has been constructed. Upon transfection into a hamster fibroblast cell line K12, the hybrid gene exhibits cell-cycle-dependent expression. Deletion analysis reveals that the region important for cell-cycle regulation is within -441 to -63 nucleotides from the transcriptional initiation site. This region (-441 to -63) also confers cell-cycle regulation to the herpes simplex virus thymidine kinase (HSVtk) promoter, which is not expressed in a cell-cycle manner. We conclude that the -441 to -63 sequence within the human TK promoter is important for cell-cycle-dependent expression.