International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1) pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE
International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1) pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE
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DOI：
10.1128/cvi.00278-15
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发表时间：
2015-08-01
影响因子：
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通讯作者：
Van Kerkhove, Maria D.
中科院分区：
文献类型：
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作者：
Laurie, Karen L.;Engelhardt, Othmar G.;Van Kerkhove, Maria D.
微量中和测定通常用于检测流感病毒抗体，并且在世界范围内使用多种方案。这些方案在测定的孵育时间以及具体步骤的顺序上有所不同，甚至在方案内，各个实验室也经常进行进一步的调整。这些方案变化对流感血清学数据的影响尚不清楚。因此，CONSISE 实验室工作组使用 A(H1N1) pdm09、A(H3N2) 和 A(H5N1) 病毒对 2 天酶联免疫吸附测定 (ELISA) 和 3 天血凝 (HA) 微中和 (MN) 方案进行了实验室比较。各个实验室使用不同的血清组多次执行这两种测定方案。来自世界各地的 13 个实验室参与其中。在每个实验室内，在测定之间比较不同测定方案的血清样品滴度，以确定每个测定的灵敏度，并在重复之间进行比较，以评估每个实验室的每个方案的再现性。大多数实验室使用两种测定方案获得的结果具有良好的相关性，表明这些测定可以互换用于检测本研究中包含的甲型流感病毒的抗体。重要的是，参与实验室已将其方法与 CONSISE 共识 2 天 ELISA 和 3 天 HA MN 检测方案进行了调整，以便将来能够更好地关联这些检测。
The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1) pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HA MN assay protocols to enable better correlation of these assays in the future.