"Off-on" signal amplification strategy amperometric immunosensor for ultrasensitive detection of tumour marker
"Off-on" signal amplification strategy amperometric immunosensor for ultrasensitive detection of tumour marker
复制标题
用于肿瘤标志物超灵敏检测的“关-开”信号放大策略安培免疫传感器
DOI：
10.1016/j.bios.2019.03.013
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发表时间：
2019-05-01
影响因子：
12.6
通讯作者：
Ma, Zhanfang
中科院分区：
文献类型：
--
作者：
Wang, Huiqiang;Ma, Zhanfang
降低背景信号是提高电流型免疫传感器灵敏度的有效方法，而电流型免疫传感器在癌症检测中具有重要意义。本文利用分子筛咪唑骨架-氢醌-牛血清白蛋白(ZIF-8-HQ-BSA)合成了一种新型的免疫传感器探针。在该探针的基础上，在免疫传感器中应用了一种“关-开”的策略来放大AI，即电流信号“关和开”。由于ZIF-8和BSA表现出较差的导电性，ZIF-8-HQ-BSA探针最初显示的电流信号很少。为了放大电流信号“开启”，用盐酸处理ZIF-8分子筛，使其结构断裂，释放出对苯二酚被聚苯胺水凝胶底物吸收。对苯二酚能产生很强的信号，对过氧化氢也表现出很强的催化作用，使信号进一步增强。该方法用于人血清中细胞角蛋白抗原21.1(CYFRA21-1)的检测，线性范围为0.1pgmL(-1)~100 ng(-1)，检出限为0.65pgmL(-1)(S/N=3)，与电化学发光免疫分析法具有良好的一致性。因此，本文所讨论的安培免疫传感平台在医疗保健监测、临床诊断和生物医学设备中具有很高的应用潜力。
Reducing background signal is an effective method to improve the sensitivity of amperometric immunosensors, which are significant in the detection of cancer. Herein, a novel immunosensor probe was synthesised using zeolitic imidazolate framework-hydroquinone-bovine serum albumin (ZIF-8-HQ-BSA). Based on the probe, an "off-on" strategy was applied in the immunosensor to amplify AI, the current signal "turn off and on". The ZIF-8-HQ-BSA probe initially showed little current signal since ZIF-8 and BSA exhibit poor conductivity. To amplify the current signal "turn on", the structure of ZIF-8 was broken after treatment with HCl, releasing hydroquinone to be absorbed by the polyaniline hydrogel substrate. Hydroquinone can produce a strong signal and also exhibits strong catalytic ability for H2O2 to further enhance the signal. Following this method, the immunosensing platform was used to detect cytokeratins antigen 21.1 (CYFRA21-1) in human serum and revealed a wide liner range from 0.1 pg mL(-1) to 100 ng(-1) with a low detection limit of 0.65 pg mL(-1) (S/N = 3), demonstrating good consistency with an electrochemiluminescence immunoassay. Thus, the amperometric immunosensing platform discussed in this work has high potential for application in healthcare monitoring, clinical diagnostics, and biomedical devices.