CLASSIFICATION AND CHARACTERIZATION OF HETEROTROPHIC MICROBIAL COMMUNITIES ON THE BASIS OF PATTERNS OF COMMUNITY-LEVEL SOLE-CARBON-SOURCE UTILIZATION
CLASSIFICATION AND CHARACTERIZATION OF HETEROTROPHIC MICROBIAL COMMUNITIES ON THE BASIS OF PATTERNS OF COMMUNITY-LEVEL SOLE-CARBON-SOURCE UTILIZATION
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DOI：
10.1128/aem.57.8.2351-2359.1991
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发表时间：
1991-08-01
影响因子：
4.4
通讯作者：
MILLS, AL
中科院分区：
文献类型：
--
作者：
GARLAND, JL;MILLS, AL
基于四唑盐染料还原的BLOG氧化还原技术作为单一碳源利用的指标，被评估为一种快速的、群落级的异养微生物群落表征和分类方法。在含有95个独立碳源的Biolog平板中直接培养整个环境样本(水生、土壤和根际)，产生单一碳源利用的社区依赖模式。从图版数字化图像中量化的颜色反应的主成分分析揭示了土壤和河口场地内微生物生境和空间梯度之间的独特模式。原始碳源变量与主成分的相关性为群落间差异提供了函数基础。利用这项技术对微生物群落进行密集的空间和时间分析可以产生异养微生物群落的生态相关分类。
The BLOLOG redox technology based on tetrazolium dye reduction as an indicator of sole-carbon-source utilization was evaluated as a rapid, community-level method to characterize and classify heterotrophic microbial communities. Direct incubation of whole environmental samples (aquatic, soil, and rhizosphere) in BIOLOG plates containing 95 separate carbon sources produced community-dependent patterns of sole-carbon-source utilization. Principal-component analysis of color responses quantified from digitized images of plates revealed distinctive patterns among microbial habitats and spatial gradients within soil and estuarine sites. Correlation of the original carbon source variables to the principal components gives a functional basis to distinctions among communities. Intensive spatial and temporal analysis of microbial communities with this technique can produce ecologically relevant classifications of heterotrophic microbial communities.