VIP-induced alterations in cAMP and inositol phosphates in the lower esophageal sphincter.
VIP-induced alterations in cAMP and inositol phosphates in the lower esophageal sphincter.
复制标题
VIP 诱导下食管括约肌中 cAMP 和磷酸肌醇的变化。
DOI：
10.1152/ajpgi.1990.259.2.g239
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Biancani,P
中科院分区：
文献类型：
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作者：
Szewczak,SM;Behar,J;Billett,G;Hillemeier,C;Rhim,BY;Biancani,P
在血管活性肠肽（VIP）诱导的松弛过程中，在37 ℃下测量猫下食管括约肌（LES）环形平滑肌的薄组织样本中3 '，5'-环一磷酸腺苷（cAMP）、3 '，5'-环一磷酸鸟苷（cGMP）和磷酸肌醇（IP）水平。在相同温度下，将离体LES环形肌条暴露于10（-6）M VIP时，自发静息张力松弛在3-6 s内开始，在30 s时达到最大值的一半，在1 min时达到最大值。在加入10（-6）M VIP后5 s和30 s测量VIP诱导的cAMP、cGMP和IP代谢物水平的变化。在5秒cAMP水平显着增加相对于时间匹配的未刺激的控制，而肌醇1，4，5-三磷酸（1，4，5-IP 3）下降，这些变化保持不变，在30秒。暴露于10（-6）M VIP后5或30 s，cGMP水平无变化。这些数据表明VIP诱导的舒张与降低的1，4，5-IP 3以及增加的cAMP水平在时间上相关。
Adenosine 3',5'-cyclic monophosphate (cAMP), guanosine 3',5'-cyclic monophosphate (cGMP), and inositol phosphate (IP) levels were measured in thin tissue samples from the circular smooth muscle of the cat lower esophageal sphincter (LES) at 37 degrees C during vasoactive intestinal peptide (VIP)-induced relaxation. On exposure of in vitro LES circular muscle strips to 10(-6) M VIP at the same temperature, relaxation of spontaneous resting tone begins within 3-6 s, is half maximal at 30 s, and maximal at 1 min. VIP-induced changes in cAMP, cGMP, and IP metabolite levels were measured at 5 and 30 s after the addition of 10(-6) M VIP. At 5 s cAMP levels increased significantly with respect to time-matched unstimulated controls, whereas inositol 1,4,5-trisphosphate (1,4,5-IP3) decreased and these changes remained constant at 30 s. cGMP levels were unchanged at either 5 or 30 s after exposure to 10(-6) M VIP. These data suggest that VIP-induced relaxation is temporally linked to decreased 1,4,5-IP3 as well as increased cAMP levels.