Effects of miR-34c-5p on Sodium, Potassium, and Calcium Channel Currents in C2C12 Myotubes
Effects of miR-34c-5p on Sodium, Potassium, and Calcium Channel Currents in C2C12 Myotubes
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miR-34c-5p 对 C2C12 肌管钠、钾和钙通道电流的影响
DOI：
10.1007/s10571-020-00810-9
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发表时间：
2020-02-25
影响因子：
4
通讯作者：
Yin, Xiaofeng
中科院分区：
文献类型：
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作者：
Jin, Bo;Gu, Xinyi;Yin, Xiaofeng
本研究旨在探讨miR-34 c-5 p对骨骼肌细胞主要电压依赖性离子通道的影响。本研究关注miR-34 c-5 p对C2 C12成肌细胞钠、钾和钙电流的影响。将miR-34 c-5 p过表达组、敲低组和对照组分化7天，融合成肌管，并用于全细胞膜片钳记录。与对照组相比，其他两组的全细胞钠电流密度无明显变化。敲除组延迟整流钾电流密度增加（有统计学意义），全细胞钙通道电流密度无变化。在过表达组中，整流钾电流密度变化不明显，而峰值钙通道电流密度增加（过表达组为-9.23 ± 0.95 pA/pF，n= 6个细胞，对照组为-6.48 ± 0.64 pA/pF，n= 7个细胞;p< 0.05）。miR-34 c-5 p表达的变化可影响C2 C12肌管中钙和钾电压门控通道的电生理特性。miR-34 c-5 p过表达增加全细胞L型钙通道电流（伊卡，L），而miR-34 c-5 p敲低增加全细胞延迟整流钾电流（IKd）。
The aim of this study was to investigate the effects of miR-34c-5p on the main voltage-dependent ion channels in skeletal muscle cells. This study focused on the effects of miR-34c-5p on sodium, potassium, and calcium currents in C2C12 myoblasts. The miR-34c-5p overexpression group, knockdown group, and control group were differentiated for 7 days, fused into myotubes, and used for the whole-cell patch clamp recording. Compared with the control group, the whole-cell sodium current density of the other two groups had no significant changes. In the knockdown group, the delayed rectifier potassium current density was increased (statistically significant), and the whole-cell calcium channel current density did not change. In the overexpression group, the change of rectifier potassium current density was not obvious, while the peak calcium channel current density increased (− 9.23 ± 0.95 pA/pF,n= 6 cells for the overexpression group vs. − 6.48 ± 0.64 pA/pF,n= 7 cells for the control;p< 0.05). Changes in the expression of miR-34c-5p can affect the electrophysiological characteristics of calcium and potassium voltage-gated channels in C2C12 myotubes. Overexpression of miR-34c-5p increased whole-cell L-type calcium channel current (ICa,L), while miR-34c-5p knockdown increased whole-cell delayed rectifier potassium current (IKd).