Diversity of roles of protein kinase C alpha in the proliferation of Swiss 3T3 cells.
Diversity of roles of protein kinase C alpha in the proliferation of Swiss 3T3 cells.
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蛋白激酶 C α 在 Swiss 3T3 细胞增殖中的作用多样性。
DOI：
10.1042/bj3150513
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Calle,R
中科院分区：
文献类型：
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作者：
Florin-Christensen,J;Florin-Christensen,M;Meinardi,E;Calle,R
本文研究了蛋白激酶Cα（PKCα）在蛙皮素、血小板衍生生长因子（PDGF）和佛波酯（PMA）刺激Swiss 3 T3细胞DNA合成中的作用。我们发现，这种激酶被下调的细胞表现出对蛙皮素的部分抑制的促有丝分裂反应。对PDGF的反应没有改变;然而，对PMA的反应被完全抑制。最大剂量的蛙皮素和PMA组合的促有丝分裂作用大于单独的任一药剂，表明蛙皮素不完全激活PKC通路。因此，蛙皮素诱导的PKCα从胞浆到膜的移位是部分的，而PMA观察到的移位基本上是完全的。此外，暴露于PKC抑制剂Ro-31-8220对PMA反应的影响显著大于对蛙皮素的影响。我们的研究结果指出，PKCα可能发挥不同的作用，在diabetes激活的细胞：而在PMA的情况下，这种激酶的刺激可能是必要的，足以诱导增殖，它似乎是必要的，只有一个全面的反应蛙皮素，和多余的机制中触发的PDGF。
We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF.