Molecular organization and expression of the genetic locus glued in Drosophila melanogaster.
Molecular organization and expression of the genetic locus glued in Drosophila melanogaster.
复制标题
果蝇中粘合的遗传位点的分子组织和表达。
DOI：
10.1128/mcb.6.3.833-841.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Garen,A
中科院分区：
文献类型：
--
作者：
Swaroop,A;Sun,JW;Paco-Larson,ML;Garen,A
果蝇的Glued基因座在遗传学上被定义为受显性Glued突变G1影响的功能单位。基因组DNA是从3号染色体上70 C2的Glued位点区域克隆的，通过在该区域插入a P元件作为分子标记。在克隆DNA的30-内切酶跨度内检测到三个编码多聚腺苷酸化转录物的基因。最接近P元件插入位点的基因被鉴定为Glued基因，这是基于其DNA和编码的转录本中的改变，所述转录本与Gl突变和Gl的回复相关，所述回复通过突变等位基因的失活来消除显性效应。野生型Gl+基因的表达在发育过程中受到时间调节;编码的转录物的量在胚胎阶段最高，在第一和第二龄幼虫中降低，然后在第三龄和蛹阶段增加。G1+转录本对胚胎有母体贡献，这可能解释了Glued突变对早期发育的母体致死效应。组织切片中Gl +DNA与RNA的原位杂交表明，Gl+转录本几乎存在于胚胎、晚期幼虫和蛹的所有组织中。该转录物的一般分布与遗传证据一致，表明Glued基因座控制着通常必需的细胞功能（P. J. Harte和D. R. Kankel，Genetics 101：477-501，1982）。不同的Glued突变产生不同的表型效应，包括具有严重视觉缺陷的成虫、缺乏成虫盘的幼虫和早期致死。这些不同的突变体表型的胶合功能的定量变化方面进行了讨论。与G1+紧密相邻的是另一个基因，其转录方向不同，并在果蝇发育过程中与G1+协调表达。目前尚不清楚该基因是否也参与了Glued功能。
The Glued locus ofDrosophila melanogasteris genetically defined as the functional unit which is affected by the dominant Glued mutationGl. Genomic DNA was cloned from the region of the Glued locus, at 70C2 on chromosome 3, by using aPelement insertion in the region as a molecular marker. Three genes encoding polyadenylated transcripts were detected within a 30-kilobase span of the cloned DNA. The gene nearest thePelement insertion site was identified as a Glued gene on the basis of alterations in its DNA and encoded transcript associated with theGlmutation and with reversions ofGlwhich eliminate the dominant effect by inactivation of the mutant allele. Expression of the wild-typeGl+gene is temporally regulated during development; the amount of the encoded transcript is highest in the embryonic stage, decreasing in the first and second larval instars, and then increasing in the third instar and pupal stages. There is a maternal contribution of theGl+transcript to the embryo, which probably accounts for the maternal lethal effect of Glued mutations on early development. In situ hybridizations ofGl+DNA to RNA in tissue sections showed that theGl+transcript is present in virtually all tissues of the embryo, late larva, and pupa. The general distribution of this transcript is consistent with genetic evidence indicating that the Glued locus controls a generally essential cell function (P. J. Harte and D. R. Kankel, Genetics 101:477-501, 1982). Different Glued mutations produce distinct phenotypic effects, including adults with severe visual defects, larvae lacking imaginal discs, and early lethality. These diverse mutant phenotypes are discussed in terms of quantitative changes in the Glued function. Closely adjacent toGl+is another gene which is transcribed in a divergent direction and expressed coordinately withGl+throughoutDrosophiladevelopment. It remains to be determined whether this gene is also involved with the Glued function.