Prolactin and growth hormone regulate adiponectin secretion and receptor expression in adipose tissue
Prolactin and growth hormone regulate adiponectin secretion and receptor expression in adipose tissue
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DOI：
10.1016/j.bbrc.2005.04.026
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发表时间：
2005-06-17
影响因子：
3.1
通讯作者：
Billig, H
中科院分区：
文献类型：
--
作者：
Nilsson, L;Binart, N;Billig, H
脂联素是一种由脂肪组织分泌的激素，血清水平随着肥胖和胰岛素抵抗而降低。由于催乳素（PRL）和生长激素（GH）可以影响胰岛素敏感性，我们研究了这些激素对体外人体脂肪组织和啮齿类动物体内脂联素调节的影响。在体外培养的人脂肪组织中，PRL 和 GH 显着抑制脂联素分泌。此外，PRL 增加了培养的人脂肪组织中脂联素受体 1 (AdipoR1) mRNA 的表达，而 GH 则降低了 AdipoR2 的表达。在表达 GH 的转基因小鼠和表达 PRL 的雌性小鼠中，脂联素的血清水平降低。相比之下，GH 受体缺陷小鼠的脂联素水平升高，而 PRL 受体缺陷小鼠则不受影响。总之，我们首次证明了人类脂肪组织中 AdipoR1 和 AdipoR2 的基因表达，并表明它们受到 PRL 和 GH 的差异调节。 PRL 和 GH 均能减少体外人体脂肪组织和小鼠体内脂联素的分泌。血清脂联素水平降低与胰岛素抵抗有关，我们在人体组织和转基因小鼠中的数据表明脂联素在 PRL 和 GH 诱导的胰岛素抵抗中发挥作用。 (c) 2005 Elsevier Inc. 保留所有权利。
Adiponectin is a hormone secreted from adipose tissue, and serum levels are decreased with obesity and insulin resistance. Because prolactin (PRL) and growth hormone (GH) can affect insulin sensitivity, we investigated the effects of these hormones on the regulation of adiponectin in human adipose tissue in vitro and in rodents in vivo. Adiponectin secretion was significantly suppressed by PRL and GH in in vitro cultured human adipose tissue. Furthermore, PRL increased adiponectin receptor 1 (AdipoR1) mRNA expression and GH decreased AdipoR2 expression in the cultured human adipose tissue. In transgenic mice expressing GH, and female mice expressing PRL, serum levels of adiponectin were decreased. In contrast, GH receptor deficient mice had elevated adiponectin levels, while PRL receptor deficient mice were unaffected. In conclusion, we demonstrate gene expression of AdipoR1 and AdipoR2 in human adipose tissue for the first time, and show that these are differentially regulated by PRL and GH. Both PRL and GH reduced adiponectin secretion in human adipose tissue in vitro and in mice in vivo. Decreased serum adiponectin levels have been associated with insulin resistance, and our data in human tissue and in transgenic mice suggest a role for adiponectin in PRL and GH induced insulin resistance. (c) 2005 Elsevier Inc. All rights reserved.