Fluorescence Quenching Nanoprobes Dedicated to In Vivo Photoacoustic Imaging and High-Efficient Tumor Therapy in Deep-Seated Tissue
Fluorescence Quenching Nanoprobes Dedicated to In Vivo Photoacoustic Imaging and High-Efficient Tumor Therapy in Deep-Seated Tissue
复制标题
荧光猝灭纳米探针致力于体内光声成像和深部组织高效肿瘤治疗
DOI：
10.1002/smll.201403395
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发表时间：
2015-06-10
期刊：
影响因子：
13.3
通讯作者：
Xing, Da
中科院分区：
文献类型：
--
作者：
Qin, Huan;Zhou, Ting;Xing, Da
光声成像(PAI)和光声治疗(PA)在肿瘤治疗中具有广阔的应用前景。众所周知，利用高吸收系数的探针可以选择性地提高PAI靶区对比度和深层组织中PA肿瘤的治疗效率。本文报道了利用氧化石墨烯(GO)和染料的通孔堆积作用，设计了一种具有最高光热转换效率的探针。GO作为负载染料和通过荧光共振能量转移(FRET)猝灭染料荧光的基础材料，目的之一是最大限度地提高PA效率。实验证明，所设计的荧光猝灭纳米探针可以产生比染料和GO中产生的单独信号的总和更强的PA信号。展示了荧光猝灭纳米探针的潜在应用，致力于提高活体小鼠深层组织和肿瘤中靶标的PA对比度。利用荧光猝灭纳米探针在体内外治疗PA的效率均高于常用PA治疗剂。综上所述，FRET猝灭染料荧光为开发高效PA探针提供了有效手段。荧光猝灭纳米探针很有可能成为深层次肿瘤成像和治疗的潜在候选者。
Photoacoustic imaging (PAI) and photoacoustic (PA) therapy have promising applications for treating tumors. It is known that the utilization of high-absorption-coefficient probes can selectively enhance the PAI target contrast and PA tumor therapy efficiency in deep-seated tissue. Here, the design of a probe with the highest availability of optical-thermo conversion by using graphene oxide (GO) and dyes via - stacking interactions is reported. The GO serves as a base material for loading dyes and quenching dye fluorescence via fluorescence resonance energy transfer (FRET), with the one purpose of maximum of PA efficiency. Experiments verify that the designed fluorescence quenching nanoprobes can produce stronger PA signals than the sum of the separate signals generated in the dye and the GO. Potential applications of the fluorescence quenching nanoprobes are demonstrated, dedicating to enhance PA contrast of targets in deep-seated tissues and tumors in living mice. PA therapy efficiency both in vitro and in vivo by using the fluorescence quenching nanoprobes is found to be higher than with the commonly used PA therapy agents. Taken together, quenching dye fluorescence via FRET will provide a valid means for developing high-efficiency PA probes. Fluorescence quenching nanoprobes are likely to become a promising candidate for deep-seated tumor imaging and therapy.