Differential expression of VEGF isoforms and receptors in knee joint menisci under systemic hypoxia
Differential expression of VEGF isoforms and receptors in knee joint menisci under systemic hypoxia
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DOI：
10.1016/j.bbrc.2004.09.103
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发表时间：
2004-11-12
影响因子：
3.1
通讯作者：
Glimcher, MJ
中科院分区：
文献类型：
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作者：
Hofstaetter, JG;Saad, FA;Glimcher, MJ
血管内皮生长因子（VEGF）基因产生了几种不同的VEGF亚型，它们在表达模式以及生化和生物学特性上都有所不同。我们检测了正常生理条件下兔内侧半月板和外侧半月板中VEGF同种异构体及其受体的表达水平，以及8和24小时全身常压缺氧（13%）后的表达水平。VEGF（121）是内侧和外侧半月板中最丰富的VEGF亚型，其次是VEGF（165）、VEGF（189）和VEGF（183）。可溶性VEGF（121）和VEGF165仅在缺氧8 h时上调，而膜结合的VEGF（183）和VEGF（189）在缺氧24 h时进一步升高。正常情况下，VEGFR-2的表达水平远高于VEGFR-1，两种受体在缺氧条件下均上调。正常缺氧条件下VEGF表达水平的差异以及对缺氧的不同反应可能表明VEGF同种异构体在半月板中的不同功能。(C) 2004爱思唯尔公司版权所有。
Vascular endothelial growth factor (VEGF) gene gives rise to several distinct isoforms of VEGF, which differ in their expression patterns as well as their biochemical and biological properties. We examined the expression levels of VEGF isoforms and their receptors in the medial and lateral meniscus of rabbits under normal physiologic conditions as well their expression levels after 8 and 24 h of systemic normobaric hypoxia (13%). VEGF(121) is the most abundant VEGF isoform in the medial and lateral meniscus, followed by VEGF(165), VEGF(189), and VEGF(183). While the soluble VEGF(121) and VEGF165 are only upregulated at 8 h of hypoxia, the membrane-bound VEGF(183) and VEGF(189) are further increased at 24 h. VEGFR-2 is expressed at a much higher level than VEGFR-1 under normal conditions, and both receptors are upregulated under hypoxia. Differential expression levels under normoxia as well as a differential response to hypoxia may indicate different functions of VEGF isoforms in the meniscus. (C) 2004 Elsevier Inc. All rights reserved.