Wide-Field Surface-Enhanced Coherent Anti-Stokes Raman Scattering Microscopy
Wide-Field Surface-Enhanced Coherent Anti-Stokes Raman Scattering Microscopy
复制标题
宽视场表面增强相干反斯托克斯拉曼散射显微镜
DOI：
10.1021/acsphotonics.1c02015
复制
发表时间：
2022
期刊：
影响因子：
7
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Zong, Cheng;Cheng, Ran;Chen, Fukai;Lin, Peng;Zhang, Meng;Chen, Zhicong;Li, Chuan;Yang, Chen;Cheng, Ji-Xin
表面增强拉曼散射(SERS)光谱已被广泛应用于生物、化学和材料的研究。然而，逐点SERS映射是耗时的，大规模成像需要几分钟到几个小时。在这里，我们报道了一种广场表面增强相干反斯托克斯拉曼散射(WISE-CARS)显微镜，用于监测活细胞中的纳米标记和代谢分子的无标记检测。WISE-CARS显微镜的成像速度为120fps，视场为130μm×130μm。通过飞秒激光的光谱聚焦，在0.5 S内可以获得60帧的高光谱WISE-CARS堆栈，其中100多万个拉曼光谱被并行记录，光谱分辨率为10 cm-1。作为应用，我们展示了活细胞中纳米标记的时延、三维(3D)WISE-CARS成像以及腺嘌呤释放的无标记检测。金星。
Surface-enhanced Raman scattering (SERS) spectroscopy has been used extensively to study biology, chemistry, and materials. However, point-by-point SERS mapping is time-consuming, taking minutes to hours for large-scale imaging. Here, we report a wide-field surface-enhanced coherent anti-Stokes Raman scattering (WISE-CARS) microscope for monitoring nanotags in live cells and label-free detection of metabolic molecules. The WISE-CARS microscope achieves an imaging speed of 120 fps for a field of view of 130 μm × 130 μm. By spectral focusing of femtosecond lasers, a hyperspectral WISE-CARS stack of 60 frames can be acquired within 0.5 s, where over 1 million Raman spectra are parallelly recorded with a spectral resolution of 10 cm–1. As applications, we demonstrate time-lapse, three-dimensional (3D) WISE-CARS imaging of nanotags in live cells as well as label-free detection of adenine released fromS. aureus.