Tumor hypoxia and expression of c-met in cervical cancer
Tumor hypoxia and expression of c-met in cervical cancer
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DOI：
10.1016/j.ygyno.2006.07.040
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发表时间：
2007-01-01
影响因子：
4.7
通讯作者：
Hoeckel, Michael
中科院分区：
文献类型：
--
作者：
Leo, Cornelia;Horn, Lars-Christian;Hoeckel, Michael
目标.缺氧通过促进转移的发展和增加侵袭性而促进恶性进展。控制癌细胞生长、侵袭和转移的一个关键调节因子是生长因子受体c-met。因此，本研究的目的是探讨c-met原癌基因在宫颈癌中的表达与肿瘤内缺氧水平和临床病理参数的关系。应用Eppendorf电极对43例宫颈癌患者进行了肿瘤内PO 2测定和活检。组织随后用抗c-met抗体进行免疫组织化学分析。c-met在72%的宫颈癌中表达。在低分化肿瘤中有显著更强的表达（F=0.4，p=0.008）。此外，c-met表达与喷雾样浸润模式显著相关（P=0.008）。而c-met表达与肿瘤内缺氧、pT分期、FIGO分期、淋巴管间隙受累、肿瘤大小及总生存期均无明显关系。虽然c-met在体外被证明是缺氧诱导的，但我们的研究结果表明，它不是缺氧对宫颈癌临床结局有害影响的介导者。(c)2006年爱思唯尔公司All rights reserved.
Objectives. Hypoxia enhances malignant progression by promoting the development of metastases and increasing invasiveness. One key regulator that controls growth, invasion and metastasis in cancer cells is the growth factor receptor c-met. The aim of this study, therefore, was to investigate the expression of the c-met protooncogene in cervical cancers in relation to intratumoral hypoxia levels and to clinico-pathological parameters.Methods. 43 Patients with cervical cancer were subjected to intratumoral PO2 measurement with the Eppendorf electrode and biopsies were taken. The tissue was subsequently analyzed by immunohistochemistry with an anti-c-met antibody.Results. c-met was expressed in 72% of cervical cancers. There was a significantly stronger expression in poorly differentiated tumors (F=0.4, p=0.008). Furthermore, c-met expression was significantly associated with a spray-like pattern of invasion (P=0.008). However, there was no significant relationship between c-met expression and intratumoral hypoxia, pT stage, FIGO stage, lymphovascular space involvement, tumor size or overall survival.Conclusions. Although c-met has been shown to be hypoxia-induced in vitro, our results suggest that it is not the mediator of deleterious effects of hypoxia on clinical outcome in cervical cancer. (c) 2006 Elsevier Inc. All rights reserved.