Morphologic basis for a pore-pathway in mammalian stratum corneum.
Morphologic basis for a pore-pathway in mammalian stratum corneum.
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DOI：
10.1159/000211511
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发表时间：
1997-07
期刊：
影响因子：
--
通讯作者：
G. Menon;P. Elias
中科院分区：
文献类型：
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作者：
G. Menon;P. Elias
尽管先前的形态学研究表明极性和非极性材料均通过细胞旁途径渗透穿过角质层（SC），但通过这些异质域的实际途径尚不清楚。我们在基础条件下和/或通过闭塞、载体增强剂、脂质合成抑制剂、超声导入和离子电渗疗法增强渗透后，将亲水性和疏水性示踪剂体内应用到小鼠皮肤。使用四氧化钌、钌红加四氧化锇、锇蒸气原位沉淀和微波后固定方法来可视化渗透路径。示踪剂总是定位于嵌入细胞外层状膜系统内的离散腔隙域，无论其极性或增强方法如何。此外，虽然腔隙域在基础条件下仍然不连续，但它们似乎通过渗透增强获得了结构连续性。这些结果表明细胞外腔隙结构域包含极性和非极性分子穿过 SC 的孔道。
Although prior morphologic studies have shown that both polar and nonpolar materials permeate across the stratum corneum (SC) via a paracellular route, the actual pathway through these heterogeneous domains is unknown. We applied hydrophilic and hydrophobic tracers in vivo to murine skin under basal conditions and/or after permeation enhancement with occlusion, vehicle enhancers, a lipid synthesis inhibitor, sonophoresis, and iontophoresis. Ruthenium tetroxide, ruthenium red plus osmium tetroxide, in situ precipitation with osmium vapor, and microwave postfixation methods were used to visualize penetration pathways. Tracers invariably localized to discrete lacunar domains embedded within the extracellular lamellar membrane system, regardless of their polarity or the enhancement method. Moreover, while the lacunar domains remained discontinuous under basal conditions, they appeared to gain structural continuity with permeation enhancement. These results indicate that extracellular lacunar domains comprise a pore pathway for penetration of polar and nonpolar molecules across the SC.