Extracellular Dopamine, Norepinephrine, and Serotonin in the Nucleus Accumbens of Freely Moving Rats During Intracerebral Dialysis with Cocaine and Other Monoamine Uptake Blockers
Extracellular Dopamine, Norepinephrine, and Serotonin in the Nucleus Accumbens of Freely Moving Rats During Intracerebral Dialysis with Cocaine and Other Monoamine Uptake Blockers
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DOI：
10.1046/j.1471-4159.1996.66020559.x
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发表时间：
1996-02
影响因子：
4.7
通讯作者：
Ming‐Ya Li;Q. Yan;L. L. Coffey-L.;M. Reith
中科院分区：
文献类型：
--
作者：
Ming‐Ya Li;Q. Yan;L. L. Coffey-L.;M. Reith
摘要：通过透析探针将单胺摄取阻滞剂（0.1-1,000 µM）局部应用到自由活动大鼠的伏核中，并测量细胞外多巴胺、去甲肾上腺素和血清素的浓度。选择性多巴胺摄取阻滞剂GBR 12935优先增加多巴胺，对去甲肾上腺素影响很小，而选择性5-羟色胺摄取阻滞剂氟西汀优先增加5-羟色胺输出。相比之下，选择性去甲肾上腺素摄取阻滞剂地昔帕明和尼索西汀不仅可以增强去甲肾上腺素，还可以显着增强血清素和多巴胺。可卡因会增加所有三种胺的含量，对多巴胺和血清素的影响最大。正如我们之前对腹侧被盖区的研究一样，当所有阻滞剂数据放在一起时，多巴胺和去甲肾上腺素输出之间存在正相关。目前的结果表明，在伏隔核局部施用可卡因后，去甲肾上腺素的增加而非血清素的增加对多巴胺的增强有贡献。
Abstract: Monoamine‐uptake blockers were applied focally (0.1–1,000 µM) through a dialysis probe in the nucleus accumbens of freely moving rats, and the extracellular concentrations of dopamine, norepinephrine, and serotonin were measured. The selective dopamine‐uptake blocker GBR 12935 increased dopamine preferentially with only a small effect on norepinephrine, whereas the selective serotonin‐uptake blocker fluoxetine increased serotonin output preferentially. In contrast, the selective norepinephrine‐uptake blockers desipramine and nisoxetine enhanced not only norepinephrine, but also serotonin and dopamine appreciably. Cocaine increased all three amines with the greatest effects on dopamine and serotonin. As in our previous study on the ventral tegmental area, there was a positive association between dopamine and norepinephrine output when all blocker data were taken together. The present results suggest a contribution of the increase in norepinephrine, but not serotonin, to the enhancement of dopamine after cocaine applied focally in the nucleus accumbens.