Bioligand interaction assay by flow injection absorptiometry.
Bioligand interaction assay by flow injection absorptiometry.
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DOI：
10.1021/ac9706537
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发表时间：
1997-12
影响因子：
7.4
通讯作者：
J. Ruzicka;A. Ivaska
中科院分区：
文献类型：
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作者：
J. Ruzicka;A. Ivaska
介绍了一种新的基于流动注射技术和紫外-可见吸收光谱法相结合的生物配体相互作用的研究方法。通过监测各种蛋白质与蛋白质G的结合以及各种胰岛素类似物与单克隆抗胰岛素抗体的结合来证明该概念，从而提供与基于表面等离子体共振（SPR）的技术的性能的比较。生物配体相互作用流动注射吸收法的优点是速度快，成本低，不需要再生的固体基质，和光谱分辨率与SPR传感不可用。据信，这种技术将对整个生物传感器领域产生影响，因为它允许在真实的时间内以高灵敏度在宽光谱范围内同时监测标记的以及未标记的物质。
A novel technique for the study of bioligand interactions based on combining flow injection on renewable surfaces with UV-visible absorptiometry is introduced. The concept is proven by monitoring the binding of various proteins to protein G and the binding of various insulin analogs to a monoclonal anti-insulin antibody, thus providing a comparison with the performance of surface plasmon resonance (SPR)-based techniques. The advantages of the bioligand interaction flow injection absorptiometry approach are speed, low cost, no need for regeneration of solid substrate, and spectral resolution not available with SPR sensing. It is believed that this technique will have an impact on the entire biosensor field since it allows simultaneous monitoring of labeled as well as nonlabeled species in real time over a wide spectral range with high sensitivity.